茶叶水提物对猪源耐药大肠杆菌的抑制作用  

Inhibitory Effects of Tea Water Extracts on Antibiotic-resistant Isolates of Escherichia coli from Swine

作　　者：杨建英[1,2] 郭小权[3] 肖莉春[1,3] 黄名钱[1,3] 孔祥峰[1] 

机构地区：[1]中国科学院亚热带农业生态研究所中国科学院亚热带农业生态过程重点实验室,长沙410125 [2]河南科技大学医学技术与工程学院,洛阳471003 [3]江西农业大学动物科学技术学院,南昌330045

出　　处：《天然产物研究与开发》2016年第12期1959-1964,共6页Natural Product Research and Development

基　　金：国家科技支撑计划(2012BAC17B0102);中科院知识创新工程重要方向项目(KZCX2-EW-411);湖南省战略性新兴产业科技攻关项目(21014GK1007)

摘　　要：本研究旨在考察茶叶水提物对耐药大肠杆菌的抑制作用，为大肠杆菌病的防治提供依据。采用体外抑菌试验测定红茶水提物和绿茶水提物对猪源耐药大肠杆菌分离株的抑制活性，然后观察具有较强抑菌活性的绿茶水提物对大肠杆菌攻毒小鼠的保护作用。结果表明，随着浓度的增加，两种茶叶水提物的抑菌活性增强；红茶水提物和绿茶水提物对13株大肠杆菌分离株的MIC值分别为62．5-250.0mg／mL、62．5-125．0mg／mL，提示绿茶水提物的抑菌活性较强；两种茶叶水提物对13个大肠杆菌分离株的抑制活性强弱表现出一定的差异。攻毒后48h，攻毒组小鼠全部死亡，绿茶水提物组小鼠的存活率为20％；绿茶水提物组小鼠的十二指肠和空肠肠绒毛高度与对照组比较差异不显著，而显著大于攻毒组（P〈0．05），提示绿茶水提物对大肠杆菌攻毒小鼠的肠道损伤具有一定缓解作用。上述结果提示，绿茶水提物对大肠杆菌具有很好的抑制作用，可作为防治大肠杆菌病的备选生物活性物质之一。This study was conducted to investigate the inhibitory effects of tea water extracts against antibiotic-resistant Escherichia coli（ E. coli） in order to provide information for the prevention and treatment of colibacillosis. The inhibitory activities of water extracts from red tea and green tea against antibiotic-resistant E. coli isolates from swine were deter- mined by in vitro antibacterial test. The protective effect of water extracts of green tea with stronger inhibitory activity on mice challenged by antibiotic-resistant E. coli were observed in vivo. Results showed that the inhibitory activities against E. coli of two water extracts of tea increased with the increasing of their concentrations. The minimum inhibitory concen- trations of water extracts of red tea and green tea against 13 tested E. coli strains were 62.5-250.0 and 62.5-125.0 mg/ mL, respectively,which suggested that the water extracts of green tea presented stronger antibacterial activity. Two water extracts of tea presented different antibacterial activities against different strains of E. coll. At 48-h post-challenge, all mice in the challenged group were dead ,while survival rate of mice in the water extracts of green tea group was 20% ;the villus height of duodenum and jejunum of mice in the water extracts of green tea group had no significant difference compared with the control group, while was higher （ P 〈 0.05 ） compared with the challenged group, which suggested that the water extracts of green tea presented mitigative effects to intestinal trauma caused by E. eoli challenge. These findings in- dicated that water extracts of green tea presented stronger inhibitory effects against antibiotic-resistant E. coli, which could be used as an alternative bioactive substance for the prevention and treatment of colibacillosis.

关 键 词：茶叶水提物 大肠杆菌 抑菌活性 保护作用 

分 类 号：S816.7[农业科学—饲料科学] S852.61[农业科学—畜牧学]