腹泻树鼩肠道阿米巴原虫携带情况调查  

Survey on Intestinal Amoeba in Diarrhea Tree Shrews

作　　者：宋庆凯 苗雨润[1] 尹博文[1] 陈玲霞[1] 代解杰[1] 孙晓梅[1] 

机构地区：[1]中国医学科学院/北京协和医学院医学生物学研究所树胸种质资源中心,云南省重大传染病疫苗研发重点实验室,昆明650118

出　　处：《实验动物与比较医学》2016年第6期415-418,共4页Laboratory Animal and Comparative Medicine

基　　金：国家科技支撑计划（2014BA101801）,云南省联合支持国家计划项目（2015GA009）

摘　　要：目的调查腹泻树鼩肠道中阿米巴原虫携带情况，查找可能导致树鼩腹泻的寄生虫类病原，为实验树鼩的寄生虫质量控制提供依据。方法采集78只腹泻树鼩新鲜粪便和全血，分离血清，其中野生来源样本45份，人工繁育样本33份。分别采用生理盐水涂片法、碘液染色法进行阿米巴原虫形态学检测，采用ELISA方法进行粪便中阿米巴抗原和血清中IgG抗体检测。结果形态学镜检结果显示，野生来源和人工繁育腹泻树鼩肠道中阿米巴原虫携带率分别为42．22％（19／45）、33．33％（11／33）；经ELISA检测粪便中阿米巴抗原，阿米巴原虫携带率分别为64．44％（29／45）、42．42％（14／33）。采用ELISA粪便抗原检出率高于形态学镜检法。经ELISA检测血清IgG抗体阳性率分别为66．67％（30／45）、63．63％（21／33）。结论腹泻树鼩对阿米巴原虫的携带率较高，该原虫可能是导致树询腹泻的病原之一。日常饲养过程中应加强阿米巴病的监测和防治，防止肠道寄生虫病的传播。Objective To investigate the infection status of intestinal Amoeba in diarrhea tree shrews from the center of germplasm resources, to find out the cause of diarrhea, and to provide useful reference and support for prevention and treatment of parasitic infection in tree shrews. Methods Totally 78 samples were collected from the fecal and blood of diarrhea tree shrews, and then were detected by the normal saline direct smear method and iodine solution staining, meanwhile stool antigen and IgG antibody in serum were detected by ELISA of Amoeba. Results The results showed that the positive infection rate were 42.22%（19/45） of wild tree shrews, 33.33%（11/33） of breeding of tree shrews by direct smear and iodine staining method. The infectious rates of E. histolytica were 64.44%（29/45） and 42.42%（14/33） respectively when detected the stool antigen by ELISA. The positive rate of Amoeba IgG were 66.67% （30/45） and 63.63%（21/33） by ELISA detection. Conclusions Amoeba infection rate is rather high in tree shrews, and it may be one of the pathogens of diarrhea tree shrews. In routine breeding should be strengthened in the process of testing control the spread of the parasite.

关 键 词：树鼩 阿米巴原虫 形态学检测 ELISA检测 

分 类 号：R-332[医药卫生] R531.1