食源性产超广谱β-内酰胺酶大肠埃希菌的分布及其同源性研究  

Distribution and homology of foodborne-associated extended-spectrum β-lactamases-producing Escherichia coli

作　　者：宾羽琳[1] 王萌[1] 陈艳[1] 邱灿林[2] 招莉 钟启丽 BIN Yu-lin WANG Meng CHEN Yan QIUCan-lin ZHAOLi ZHONGQi-li(Medical Col- lege of Shaoguan University, Shaoguan 512026, China Shaoguan Center for Disease Control and Prevention, Shaoguan 512026, China)

机构地区：[1]韶关学院医学院,广东韶关512026 [2]韶关市疾病预防控制中心,广东韶关512026

出　　处：《中国感染控制杂志》2016年第12期907-912,共6页Chinese Journal of Infection Control

基　　金：韶关市科技局科技研究资金(2014CX-K280)

摘　　要：目的了解不同食源性传播介质中产超广谱β-内酰胺酶(ESBLs)大肠埃希菌的分布情况及同源性。方法从2014—2015年韶关市不同来源的食源性样本中分离产ESBLs大肠埃希菌,采用脉冲场凝胶电泳(PFGE)技术和Bio Numerics软件对菌株进行分型。结果 347份不同来源的食源性样本中检出11株致腹泻大肠埃希菌和29株产ESBLs大肠埃希菌。PFGE图谱分析,29株菌可以分为21个聚类群,其中聚类A包含7株菌(J2、J3、J4、J7、J9、B4、S3),为优势的PFGE带型,聚类B有2株菌(J6、J8),其余菌株带型为分散克隆。3株健康从业人员分离菌(J2、J7、J9)相似系数为100%,生活饮用水分离菌(S3)与腹泻患者分离菌(B4)的相似系数为97.1%,S3菌与4株健康从业人员分离菌(J2、J3、J7、J9)相似系数均>90.0%。结论韶关市食源性产ESBLs大肠埃希菌在食品、水、动物和人群中广泛分布,并可能通过食物链相互传播,应加强监测,防止进一步扩散。Objective To study the distribution and homology of foodborne-associated extended-spectrumβ-lacta-mases （ESBLs）-producing Escherichiacoli （E. coli）.Methods ESBLs-producing E. coli were isolated from differ-ent food specimens in Shaoguan from 2014 to 2015,strains were typed with pulsed-field gel electrophoresis （PFGE） and BioNumerics software.Results 1 1 strains of diarrhea-causing E. coli and 29 strains of ESBLs-producing E. co-li were isolated from 347 different sources of food specimens. PFGE analysis showed that 29 strains could be divided into 21 cluster groups,group A was predominant pattern,which included 7 strains（J2,J3,J4,J7,J9,B4,S3）, group B included 2 strains （J6,J8）,the other strains were sporadic clones. Similarity coefficient （SC）of 3 strains （J2,J7,J9）from health practitioners was 100% ,SC of strains from drinking water and patients with diarrhea （B4） was 97. 1% ,SC of S3 strain and 4 strains （J2,J3,J7,J9 ）from health practitioners were all〉90. 0% . Conclusion Foodborne-associated ESBLs-producing E. coli are widely distributed in food,water,animal,and pop-ulations,and can be transmitted through food chain,surveillance should enhanced to avoid further spread.

关 键 词：食源性 超广谱β-内酰胺酶 ESBLS 大肠埃希菌 脉冲场凝胶电泳 PFGE 聚集性 

分 类 号：R183.4[医药卫生—流行病学] R378.21[医药卫生—公共卫生与预防医学]