狄诺塞麦对去卵巢大鼠股骨干骺端骨缺损修复影响的实验研究  

Experimental study on the effects of denosumab on femoral metaphyseal defect repair in ovariectomized rats

作　　者：许九生[1] 荔婷婷 刘辉[1] 

机构地区：[1]河南科技大学第一附属医院骨科,河南洛阳471000 [2]河南科技大学附属新区医院肝脏外科,河南洛阳471000

出　　处：《中国骨质疏松杂志》2016年第12期1535-1539,共5页Chinese Journal of Osteoporosis

摘　　要：目的研究狄诺塞麦(DMAb)对去卵巢大鼠股骨干骺端骨缺损修复的影响。方法 40只健康雌性SD大鼠随机行假手术(Sham,n=15)和切除双侧卵巢(OVX,n=25)手术,术后12 w每组各取5只大鼠处死取股骨行双能X线骨密度仪检测来确定骨质疏松的建立情况,随后各组大鼠在双侧股骨干骺端建立3 mm圆形缺损,术后随机分成3组:Sham、OVX组及DMAb组。术后第一天DMAb组给予DMAb皮下注射一次,剂量为60 mg,正常饲养12 w后所有大鼠处死取股骨行Micro-CT、骨生物力学、组织切片检测。结果和OVX组、Sham组股骨干骺端缺损愈合相比,DMAb组大鼠股骨干骺端缺损区域有较高的最大载荷、骨密度(BMD)、骨体积分数(BV/TV)、骨小梁厚度(Tb.Th)、骨小粱数量(Tb.N)、连接密度(Conn.D)、骨矿化沉积率(MAR)和较低的骨小粱分离度(Tb.Sp),且各组之间比较差异有统计学意义(P<0.05)。结论 DMAb通过增加骨量,促进骨组织矿化及提高骨骼强度来加速去势大鼠股骨干骺端骨缺损的修复。Objective This study was designed to investigate the impact of Denosumab（DMAb） on the healing of femoral metaphyseal bone defects in OVX rats. Methods After bilateral ovariectomy（n = 25） or sham operation（n = 15）,12 weeks were allowed to pass before defect surgery for the establishment of standard osteoporotic animal models. Then five randomly selected OVX rats and five sham-operated ones were euthanized,and the femoral was harvested for BMD evaluation to confirm the establishment of osteoporotic animal model. Then all animals from the OVX group were randomly divided into three groups： OVX,DMAb and Sham group. DMAb group received a single dose of DMAb 60 mg/kg until death at 12 weeks. The femurs of rats were harvested for evaluation. The results of treatment for osteoporosis were evaluated by micro-computerized tomography,histology and biomechanics. Results Compared with OVX and Sham groups,the defect area of the DMAb group had higher BMD,BV/TV,Tb. Th,Tb. N,Conn. D,bone mineral apposition rates,strength and lower Tb. Sp. Conclusion These results seemed to indicate that DMAb can heal ovariectomized-induced osteoporosis defects by increasing bone mass,mineralization and strength.

关 键 词：去势大鼠 骨质疏松 骨缺损 狄诺塞麦 骨矿化 骨密度 

分 类 号：R-332[医药卫生] R965.1