RPL23-siRNA干扰片段的构建及筛选  

Construction and screening of RPL23-siRNA interference fragments

作　　者：彭文苗 张志敏[1] 胡萌[1] 余丽芳[1] 章必成[1] 饶智国[1] 秦传蓉 

机构地区：[1]中国人民解放军武汉总医院肿瘤科,武汉430070 [2]湖北中医药大学临床医学院

出　　处：《实用肿瘤学杂志》2016年第6期487-491,共5页Practical Oncology Journal

基　　金：湖北省自然科学基金(2014CFC1049);中国博士后科学基金(2014T70977)

摘　　要：目的构建并筛选RPL23-siRNA干扰片段,为RPL23在胃癌防治中作用的研究提供基础。方法利用RNA干扰技术选择3条靶序列,通过脂质体转染化学合成的RPL23-siRNA干扰片段,用Real-time PCR及Western blot方法鉴定RPL23-siRNA对胃癌MKN45细胞中RPL23的干扰效率。结果实验分为5组,与Normal cell组、RPL23 control组相比,筛选出的3个RPL23-siRNA片段转染后,细胞中RPL23 mRNA及蛋白表达均明显下降(P<0.01),多重比较发现,RPL23-siRNA1组干扰效率明显高于RPL23-siRNA2、RPL23-siRNA3组(P<0.01)。结论成功构建并筛选出RPL23-siRNA干扰片段,为后续研究提供了实验基础。Objective To construct and screen out the RPL23-siRNA interference fragments,providing the basis for the following experiments about the correlation with RPL23 and gastric cancer.Methods The RPL23- siRNA,synthesized chemically through lipofection,were selected from three target sequences by RNA interference and detected by real- time PCR and Western blot.Results Compared with normal cell group and RPL23 control group,the mRNA and protein expression of RPL23 in the other 3 interference groups were significantly decreased（P〈0.01）.Multiple comparisons showed that the interference efficiency of RPL23- siRNAl group was significantly higher than that of RPL23- siRNA2 group and RPL23- siRNA3 group（P〈0.01）.Conclusion The RPL23- siRNA interference fragment can be successfully constructed and screened out,which provides the basis for the following experiments.

关 键 词：核糖体蛋白L23 小干扰RNA RNA干扰 

分 类 号：R735.2[医药卫生—肿瘤]