糖原合成激酶3β对骨肉瘤细胞迁移和侵袭的影响  

Effects of glycogen synthase kinase-3β on migration and invasion of human osteosarcoma cells

作　　者：麦威[1] 孔令宇[1] 宋春雨[1] 邢志超[1] 王果元[1] 曲国蕃[1] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院骨科,哈尔滨150081

出　　处：《实用肿瘤学杂志》2016年第6期492-496,共5页Practical Oncology Journal

基　　金：黑龙江省科技厅科研课题(ZD201109);国家自然科学基金青年基金项目(81302337);哈医大附属肿瘤医院海燕科研基金(JJZD2014-04)

摘　　要：目的研究糖原合成激酶3β(GSK3β)在骨肉瘤侵袭、转移中的作用。方法通过Western blot法检测骨肉瘤患者肿瘤组织及骨肉瘤细胞中GSK3β的表达和磷酸化水平;使用两种GSK3β小分子抑制剂处理骨肉瘤细胞,通过细胞迁移及侵袭实验检测GSK3β活性受抑制对骨肉瘤细胞迁移、侵袭的影响。结果 GSK3在骨肉瘤细胞中过表达且存在活性调节异常;在转移及非转移骨肉瘤患者肿瘤组织中均可检测到GSK3表达及活性异常,尤其在转移的骨肉瘤患者肿瘤组织中;抑制内源性GSK3活性,明显降低肿瘤细胞的迁移、侵袭能力。结论GSK3β促进骨肉瘤细胞的侵袭、迁移,可能为骨肉瘤的临床治疗开辟潜在的靶点奠定理论基础。Objective To investigate the role of glycogen synthase kinase-3beta（GSK3β）on invasion and metastasis of human osteosarcoma cells.Methods Expression and phosphorylation of GSK3β were examined in osteosarcoma cell lines and tumor tissue from osteosarcoma patients by Western blot.The effects of small molecule GSK3βinhibitors on cell metastasis were detected by cell invasion assay and cell migration assay.Results Osteosarcoma cell lines showed increased GSK3β expression and abnormal activity regulation.In tumor tissue of patients with osteosarcoma metastasis and non- metastasis,GSK3β were detected expression and abnormal activity,especially in tumor tissue of the patients with osteosarcoma metastasis.Inhibition of GSK3β activity resulted in inhibiting cells invasion and migration in osteosarcoma cell line.Conclusion In this research,we demonstrated that GSK3β encouraged osteosarcoma cells metastasis.The result will open up a potential target for clinical treatment of osteosarcoma.

关 键 词：骨肉瘤 糖原合成激酶3β 迁移 侵袭 

分 类 号：R738.1[医药卫生—肿瘤]