桑螵蛸生制品对小鼠免疫功能和抗氧化能力的影响  

Influence of Immunologic Function and Oxidation Function Acted by Antis Egg-case before and after Processing

作　　者：贾坤静 艾雪[1,2,3] 贾天柱[1,2,3] 鞠成国[1,2,3] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]国家中医药管理局中药炮制原理解析重点实验室,辽宁大连116600 [3]辽宁省中药炮制工程技术研究中心,辽宁大连116600

出　　处：《辽宁中医杂志》2016年第12期2610-2613,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家中医药管理局行业专项项目(201107007)

摘　　要：目的:比较桑螵蛸生品、制品对小鼠免疫功能和抗氧化能力的影响。方法:对比桑螵蛸生品、制品对巨噬细胞的增殖率和吞噬作用,并测定巨噬细胞所产生的肿瘤坏死因子-α(TNF-α)和一氧化氮(NO)含量;经皮注射环磷酰胺建立小鼠免疫低下模型,测定小鼠血清白细胞介素-2(IL-2)、白细胞介素-4(IL-4)、免疫球蛋白M(Ig M)、免疫球蛋白G(Ig G)含量和胸腺、脾脏指数,体内外实验结合,比较桑螵蛸生品、制品对免疫功能的影响。经皮注射四氧嘧啶造成小鼠氧化损伤模型,测定小鼠血清丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的含量,并采用蛋白免疫印迹法(western-blotting)检测各组小鼠肝脏组织中SOD的表达,比较桑螵蛸生品、制品对氧化损伤的保护作用。结果:桑螵蛸各给药组均能促进巨噬细胞的增殖,并能提高其吞噬能力,促进巨噬细胞TNF-α和NO的释放,升高免疫低下小鼠血清中IL-2、IL-4、Ig M、Ig G含量和胸腺指数、脾脏指数。提高氧化损伤小鼠血清MDA、SOD、GSH-Px的含量,上调SOD蛋白表达水平。结论:桑螵蛸生品和炮制品均能增强免疫功能,并对氧化损伤具有一定的保护作用,生品的效果优于炮制品。Objective：To compare the effects of antis egg -case before and after processing in immunologic function and oxidation function. Methods ： Compare the value - added ratio, phagocytosis, the contents of TNF - α and NO of RAW264.7 affected by antis egg - case before and after processing. Divide the mice into six groups randomly. Make immunologic hypofunction model by giving the mice cyclophosphamide. Compare the contents of IL - 2, IL - 4, IgM, IgG and the thymus index and spleen index after treatment. Make oxidation damage model by giving the mice alloxan. Compare the contents of MDA, SOD and GSH - Px and detect the expression of SOD in the the liver of mice. Results ： Antis egg - case before and after processing can increase the contents of IL - 2, IL - 4, IgM, IgG, MDA, SOD and GSH - Px and rise the thymus index and spleen index. They can also increase the expres- sion of SOD. Conclusion ： Antis egg - case before and after processing can strengthen immunologic function and oxidation function. Antis egg - case before processing roles better than the ones after processing.

关 键 词：桑螵蛸 增强免疫 抗氧化 

分 类 号：R285[医药卫生—中药学]