粒毛盘菌属种间关系的分子系统学初探  

PHYLOGENETIC RELATIONSHIPS IN THE GENUS LACHNUM INFERRED FROM SEQUENCES OF ITS REGIONS AND 5.8S RIBOSOMAL DNA

作　　者：叶明[1,2] 庄文颖[1] 

机构地区：[1]中国科学院微生物研究所真菌地衣系统学重点实验室 [2]山东大学微生物技术国家重点实验室济南250100

出　　处：《菌物系统》2002年第3期340-345,共6页Mycosystema

基　　金：国家自然科学基金资助项目(30170003;39730020)

摘　　要：粒毛盘菌属包括晶杯菌科中子囊盘表面具有毛状物,毛状物全表面具有颗粒状纹饰以及外囊盘被结构为矩胞组织的部分种类,种间形态解剖结构多样,研究采用ITS15.8S rDNAITS2序列分析的方法探讨该属种间的系统发育关系。在供试的粒毛盘菌15个分类单元中,14个以89%的支持强度值相聚在一起,其中Lachnum clandestinum与 L. fuscescens关系最近,其支持强度值为100%;其次为L. controversum 与L. spartinae,其支持强度值为97%;而姊妹群L. euterpes,L. pteridophyllum,L. singerianum和L. lushanense彼此关系较远,其支持强度值均小于50%;L. sclerotii位于系统发育树的最外侧,为供试15个分类单元中距离最远的一个,结果表明粒毛盘菌属可能是多起源的。Phylogenetic relationships among species of Lachnum were studied by using nucleotide sequence analysis of the two internal transcribed spacer regions (ITS1 and ITS2) and 5.8S ribosomal DNA. The results showed that 14 of the 15 taxa of Lachnum tested were grouped together with a bootstrap value of 89% and L. sclerotii was the most distant species. L. clandestinum and L. fuscescens are closely related with 100% bootstrap value, and L. controversum and L. spartinae are of 97% bootstrap value. This suggests that Lachnum is probably polyphyletic.

关 键 词：序列分析 多起源 晶杯菌科 ITS1-5.85rDNA-ITS2 

分 类 号：Q939.32[生物学—微生物学]