同种异体骨髓间充质干细胞移植在心肌梗死后心室重建中的作用  

Allogeneic bone marrow mesenchymal stem cell transplantation for ventricular remodeling after myocardial infarction

作　　者：王娟[1] 贾合磊 吉红亮 任冬冬[1] 卢长青[1] Wang Juan Jia He-lei Ji Hong-liang Ren Dong-dong Lu Chang-qing(Department of Emergency, Second Clinical College of Henan University of Chinese Medicine, Zhengzhou 450002, Henan Province China Department of Internal Medicine, Zhengrong Hospital, Zhengzhou 450002, Henan Province, China)

机构地区：[1] 河南中医药大学第二临床医学院急诊科,河南省郑州市450002 [2] 郑州市郑荣 医院普内科,河南省郑州市450002

出　　处：《中国组织工程研究》2016年第50期7487-7493,共7页Chinese Journal of Tissue Engineering Research

摘　　要：背景:心肌梗死会导致心肌出现缺血性改变,引发心室重构的出现,成为导致死亡的重要原因。心肌梗死在中老年群体中多发,但患者自体骨髓间充质干细胞的增殖、分化能力较弱,为此需要积极的尝试予以同种异体干细胞移植,以获得更好的治疗效果。目的:探讨同种异体骨髓间充质干细胞移植在心肌梗死后心室重建中的作用机制。方法:取10只SD乳鼠与10只SD大鼠,进行骨髓间充质干细胞分离培养与鉴定。另取40只SD大鼠,随机分为4组,每组10只。模型组和乳鼠骨髓间充质干细胞移植组、大鼠骨髓间充质干细胞移植组均制备心肌梗死模型,假手术组予以假手术处理,造模后2个干细胞移植组分别予以相应的干细胞移植治疗。术后4周,检测各组大鼠的心功能;收集心脏组织标本,检测心脏梗死区胶原变化及梗死区血管密度。结果与结论:(1)心功能指标:术后4周,模型组各项指标与其他3组比较差异均有显著性意义(P<0.05);乳鼠骨髓间充质干细胞移植组和大鼠骨髓间充质干细胞移植组各组指标均优于模型组,但2组间差异无显著性意义(P>0.05);(2)心肌梗死区胶原含量:术后4周,模型组梗死区可观察到存在大量胶原组织形成,乳鼠骨髓间充质干细胞移植组和大鼠骨髓间充质干细胞移植组的胶原组织明显减少(P<0.05);(3)心肌梗死区血管密度:术后4周,假手术组梗死区血管密度均显著大于其他各组(P<0.05),乳鼠骨髓间充质干细胞移植组、大鼠骨髓间充质干细胞移植组梗死区血管密度均显著大于模型组(P<0.05);(4)结果表明,心肌梗死后实施成年鼠、乳鼠同种异体骨髓间充质干细胞移植均可以有效改善大鼠心功能,减少心肌梗死区胶原的形成,延缓梗死后心室重构。BACKGROUND：Myocardial infarction leads to ischemic changes in the myocardium, triggering the emergence of ventricular remodeling, which is an important cause of death. Myocardial infarction is a common disease in the middle-aged and elderly population, but autologous bone marrow mesenchymal stem cells from these patients exhibit a weak ability of proliferation and differentiation. Therefore, a positive attempt of allogeneic stem cel transplantation is required in order to obtain better therapeutic outcomes. OBJECTIVE：To explore the effect of al ogeneic bone marrow mesenchymal stem cel s on ventricular remodeling after myocardial infarction. METHODS：Bone marrow mesenchymal stem cel s from 10 neonatal rats and 10 adult rats were isolated, cultured and identified. Another 40 rats were randomly assigned into four groups （n=10/group）：model group, neonatal rat cel transplantation group, adult rat cel transplantation group, or sham group. Animal models of myocardial infarction were made in rats in the al groups except for the sham group in which the rats were given sham operation. Rats in the two cel transplantation groups were given the corresponding cel transplantation. Four weeks postoperatively, heart function of rats was detected in each group, and cardiac tissues were taken to detect changes in col agen formation and blood vessel density in the infarct area. RESULTS AND CONCLUSION：Four weeks after surgery, rats in the model group showed significant changes in cardiac function indexes as compared with the other groups （P〈0.05）, while compared with the model group, these cardiac function indexes improved in both two cel transplantation groups, but there was no significant difference between the two cell transplantation groups （P〉0.05）. Meanwhile, compared with the model group, significantly decreased collagen formation and increased blood vessel density were found in both two cell transplantation groups （P〈0.05）. Additionally, the vascular density of the infarct area was highest in th

关 键 词：干细胞 移植 心肌梗死 骨髓间充质干细胞 干细胞移植 同种异体 心室重建 心功能 血管生成 

分 类 号：R394.2[医药卫生—医学遗传学]