一株新的Pseudomonas thivervalensis MATH1 BTEX降解及脱氮能力  

BTEX biodegradation and its nitrogen removal potential by a newly isolated Pseudomonas thivervalensis MATH1

作　　者：曲丹[1,2] 赵勇胜[1,2] 任何军[1,2] 周睿[1,2] 

机构地区：[1]吉林大学环境与资源学院,长春130021 [2]吉林大学地下水资源与环境教育部重点实验室,长春130021

出　　处：《中国科技论文》2016年第21期2502-2508,共7页China Sciencepaper

基　　金：高等学校博士学科点专项科研基金资助项目(20120061120074);国家自然科学基金资助项目(41530636)

摘　　要：苯、甲苯、乙苯和二甲苯(统称为BTEX)普遍存在于地下水和土壤中,造成严重的环境问题,并对人类健康产生威胁。好氧反硝化BTEX降解菌Pseudomonas thivervalensis MATH1是从硝酸盐还原条件下BTEX污染的介质中分离的。该菌株对BTEX的去除速率分别为4.71、6.59、5.64、2.59mg/(L·d)。柠檬酸钠、硝酸盐和NaH2PO4促进菌株BTEX降解的最佳质量浓度分别为0.5g/L、100mg/L和96mg/L。此外,具有nirS和nosZ基因的MATH1对铵盐、硝酸盐和亚硝酸盐的去除速率分别为2.49mg/(L·h)、1.50mg/(L·h)和0.83mg/(L·h)。MATH1有助于减轻生物刺激所投加氮源的二次污染。该研究显示了使用MATH1对BTEX污染场地进行生物修复的可行性。Benzene, toluene, ethylbenzene, and xylene （BTEX） are of great environmental concern because of their widespread oc- currence in groundwater and soil, bringing threat to human health. The aerobic denitrifying BTEX-degrading bacterium Pseudo monas thivervalensis MATH1 is isolated from BTEX-contaminated sediment under nitrate-reducing conditions. The degradation rates of BTEX strain MATH1 are 4.71, 6.59, 5.64, 2.59 mg/（L · d）, respectively. The optimum concentrations sodium cit- rate, nitrate, and NaH2PO4 on improving BTEX biodegradation are 0. 5 g/L, 100 rag/L, and 0. 8 mmol/L, respectively. Moreo ver, MATH1, which has nirS and nosZ genes, the removal rates for ammonium, nitrate, and nitrite are 2.49 mg NH4^＋-N/（L · h）, 1.50 mg NO3 -N/（L · h）, and 0.83 mg NO2 N/（L · h）, respectively. MATH1 can help in mitigating the pollution caused by nitrogen amendments for biostimulation. This study highlights the feasibility of using MATH1 for the bioremediation of BTEX-contaminated sites.

关 键 词：环境工程 BTEX 生物降解 生物修复 脱氮 PSEUDOMONAS thivervalensis MATH1 

分 类 号：X172[环境科学与工程—环境科学]