Primer containing dimethylaminododecyl methacrylate kills bacteria impregnated in human dentin blocks  

作　　者：Chen Chen Lei Cheng Michael D Weir Nancy J Lin Sheng Lin-Gibson Xue-Dong Zhou Hockin HK Xu 

机构地区：[1]State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University [2]Biomaterials & Tissue Engineering Division, Department of Endodontics, Periodontics and Prosthodontics, University of Maryland Dental School [3]Biomaterials Group, Biosystems and Biomaterials Division, National Institute of Standards & Technology [4]Center for Stem Cell Biology & Regenerative Medicine, University of Maryland School of Medicine [5]Department of Mechanical Engineering, University of Maryland

出　　处：《International Journal of Oral Science》2016年第4期239-245,共7页国际口腔科学杂志（英文版）

基　　金：supported by NIH R01 DE17974 (Hockin HK Xu);a scholarship from West China School of Stomatology (Chen Chen);Program for New Century Excellent Talents in University (Lei Cheng);Youth Grant of Science and Technology Department of Sichuan Province China (2014JQ0033) (Lei Cheng);a bridge fund from University of Maryland Baltimore School of Dentistry (Hockin HK Xu);a seed grant from University of Maryland Baltimore (Hockin HK Xu)

摘　　要：Antibacterial dimethylaminododecyl methacrylate （DMADDM） was recently synthesized. The objectives of this study were to： （1） investigate antibacterial activity of DMADDM-containing primer on Streptococcus mutans impregnated into dentin blocks for the first time, and （2） compare the antibacterial efficacy of DMADDM with a previous quaternary ammonium dimethacrylate （QADM）. Scotchbond Multi-Purpose （SBMP） bonding agent was used. DMADDM and QADM were mixed into SBMP primer. Six primers were tested： SBMP control primer P, P＋2.5% DMADDM, P＋5% DMADDM, P＋7.5% DMADDM, P＋10% DMADDM, and P＋10% QADM. S. mutans were impregnated into human dentin blocks, and each primer was applied to dentin to test its ability to kill bacteria in dentinal tubules. Bacteria in dentin were collected via a sonication method, and the colony-forming units （CFU） and inhibition zones were measured. The bacterial inhibition zone of P＋10% DMADDM was 10 times that of control primer （P〈0.05）. CFU in dentin with P＋10% DMADDM was reduced by three orders of magnitude, compared with control. DMADDM had a much stronger antibacterial effect than QADM, and antibacterial efficacy increased with increasing DMADDM concentration. Dentin shear bond strengths were similar among all groups （P〉0.1）. In conclusion, antibacterial DMADDM-containing primer was validated to kill bacteria inside dentin blocks, possessing a much stronger antibacterial potency than the previous QADM. DMADDM-containing bonding agent was effective in eradicating bacteria in dentin, and its efficacy was directly proportional to DMADDM mass fraction. Therefore, DMADDM may be promisine for use in bonding agents as well as in other restorative and oreventive materials to inhibit bacteria.Antibacterial dimethylaminododecyl methacrylate （DMADDM） was recently synthesized. The objectives of this study were to： （1） investigate antibacterial activity of DMADDM-containing primer on Streptococcus mutans impregnated into dentin blocks for the first time, and （2） compare the antibacterial efficacy of DMADDM with a previous quaternary ammonium dimethacrylate （QADM）. Scotchbond Multi-Purpose （SBMP） bonding agent was used. DMADDM and QADM were mixed into SBMP primer. Six primers were tested： SBMP control primer P, P＋2.5% DMADDM, P＋5% DMADDM, P＋7.5% DMADDM, P＋10% DMADDM, and P＋10% QADM. S. mutans were impregnated into human dentin blocks, and each primer was applied to dentin to test its ability to kill bacteria in dentinal tubules. Bacteria in dentin were collected via a sonication method, and the colony-forming units （CFU） and inhibition zones were measured. The bacterial inhibition zone of P＋10% DMADDM was 10 times that of control primer （P〈0.05）. CFU in dentin with P＋10% DMADDM was reduced by three orders of magnitude, compared with control. DMADDM had a much stronger antibacterial effect than QADM, and antibacterial efficacy increased with increasing DMADDM concentration. Dentin shear bond strengths were similar among all groups （P〉0.1）. In conclusion, antibacterial DMADDM-containing primer was validated to kill bacteria inside dentin blocks, possessing a much stronger antibacterial potency than the previous QADM. DMADDM-containing bonding agent was effective in eradicating bacteria in dentin, and its efficacy was directly proportional to DMADDM mass fraction. Therefore, DMADDM may be promisine for use in bonding agents as well as in other restorative and oreventive materials to inhibit bacteria.

关 键 词：antibacterial bonding agent dental restoration dentin bond strength dimethylaminododecyl methacrylate kil]ing bacteriain dentin Streptococcus mutans 

分 类 号：R78[医药卫生—口腔医学]