黄瓜绿斑驳花叶病毒病生防菌株的分离与筛选  

Screening Bacteria for Biological Control of Cucumber Green Mottle Mosaic Virus Disease

作　　者：卫甜 李宏伟[2] 苏建坤[1] 刘建凤[1] 刘红霞[2] 

机构地区：[1]江苏里下河地区农业科学研究所,江苏扬州225007 [2]南京农业大学植物保护学院,南京210095

出　　处：《园艺学报》2016年第12期2391-2400,共10页Acta Horticulturae Sinica

基　　金：江苏省农业科技自主创新项目(CX(14)2059);国家自然科学基金项目(31371925);国家公益性行业(农业)科研专项基金项目(201303028;201003065)

摘　　要：从黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)病发病严重的田块采集黄瓜健株和病株,从不同生境(土壤、根围、叶围、茎围、根内、茎内和叶内)分离得到587株生防细菌。通过对各菌株胞外酶(葡聚糖酶、纤维素酶、蛋白酶和几丁质酶)活性及产嗜铁素活性进行测定,根据不同指标对菌株进行赋值,选择出157个菌株。进一步对其进行指纹图谱聚类分析,根据ARDRA图谱分析,筛选出47个菌株。通过47个菌株温室防效试验,发现9株菌株的防效达40%以上,其中菌株HW2的防效高达57.02%,其来源于嗜麦芽寡养单胞菌属(Stenotrophomonas maltophilia),另外8个均来源于芽孢杆菌(Bacillus sp.),其中3个为枯草芽孢杆菌(Bacillus subtilis)。对47个菌株的活性赋值和温室防效进行相关性分析,相关系数为0.83。通过试验建立了针对CGMMV筛选生防菌株的系统,该系统可以为其他病毒病害生防菌株筛选提供参考。Five hundred and eighty-seven bacterial isolates were obtained from different habitats（rhizosphere，endorhiza，surface and interior of stems，phyllosphere and endosphere）of field-grown cucumber plants and bulk soil. An assessment system was established to evaluate their biocontrol potential of 157 isolates strains according to in vitro activities of their extracellular metabolite（protease，chitinase，cellulase，glucanase and siderophores）. ARDRA analysis was performed to examine the genetic diversity of 157 isolates，of which 47 exhibiting relatively abundant genetic diversity was investigated for their biocontrol efficacy against CGMMV under greenhouse conditions. Nine bacterial isolates achieved positive biocontrol efficacy more than 40% at 30 days post pathogen inoculation（dpi）and HW2 strain can get at 57.02%. Among the 9 strains，one is from Stenotrophomonas maltophilia，others are from Bacillus sp. The conventional correlation efficient was 0.83 between the biocontrol efficacy of 47 isolates and their assessed biocontrol potential，indicating that the established assessment system was effective and can evaluate their biocontrol potential according to in vitro activities of their extracellular metabolites.

关 键 词：黄瓜 黄瓜绿斑驳花叶病毒 菌株筛选 酶活性测定 聚类分析 

分 类 号：S642.2[农业科学—蔬菜学]