姜黄素对脑缺血再灌注损伤大鼠PI3K/AKT/mTOR的影响  

Effect of curcumin on PI3K/AKT/mTOR in rats with cerebral ischemia-reperfusion injury

作　　者：兰晶[1] 潘敬芳[1] 

机构地区：[1]宜昌市中心人民医院/三峡大学第一临床医学院神经内科,宜昌湖北443000

出　　处：《重庆医学》2017年第1期36-39,共4页Chongqing medicine

基　　金：湖北省宜昌市科技基金资助项目(A11301-05)

摘　　要：目的探讨姜黄素对大鼠脑缺血再灌注损伤的作用及机制。方法通过线栓法构建大鼠脑缺血再灌注损伤模型。评估姜黄素对大鼠脑梗死范围、脑含水量、神经症状、脑组织病理形态,以及磷脂酰肌醇3-激酶(PI3K)、蛋白激酶B(AKT)、磷酸化蛋白激酶B(p-AKT)、雷帕霉素靶蛋白(mTOR)、丙二醛(MDA)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)、超氧化物歧化酶(SOD)、B细胞淋巴因子2(Bcl-2)、Bcl-2相关X蛋白(Bax)、半胱氨酸的天冬氨酸蛋白水解酶3(Caspase-3)、活化性半胱胺酸天冬氨酸蛋白水解酶3(Cleavage-Caspase-3)的表达影响。结果姜黄素对脑缺血再灌注损伤有保护作用,其可以减轻大鼠神经症状和脑组织病理形态的改变,以及脑梗死面积和脑含水量。此外,姜黄素还可以减轻MDA、Bax、Cleavage-Caspase-3、促炎症细胞因子IL-6、MCP-1和TNF-α的表达,增加PI3K、p-AKT、mTOR、Bcl-2、Caspase-3、CAT、GPX与SOD表达。结论姜黄素预处理对脑缺血再灌注有明显保护作用,该作用可能与激活PI3K/AKT/mTOR信号通路激活而抑制炎症,凋亡和氧化应激相关。Objective To explore the effect and mechanism of curcumin on rat cerebral ischemia reperfusion injury. Methods The rat model of cerebral ischemia reperfusion injury was constructed by the suture-occluded method. The effects of curcumin on cerebral infarction range,cerebral water content, neurological symptoms, cerebral histopathological morphology and expressions of PI3K,AKT,p-AKT,m-TOR,MDA,CAT,GPX,SOD,Bcl-2,Bax,Caspase-3 and Cleavage-Caspase-3 were evaluated. Results Curcumin had the protective effect on cerebral ischemia reperfusion injury, could alleviate the neurological symptoms, decreased the cerebral tissue pathological morphological changes and cerebral water content, in addition, which could alleviate the expressions of MDA, Bax, Cleavage-Caspase-3, IL-6, MCP-1 and TNF-α and increased the expressions of PI3K, p-AKT, roTOR, Bcl-2, Caspase-3, CAT,GPX and SOD. Conclusion The curcumin pretreatment has the significantly protective effect on cerebral ischemia-reperfusion injury,which may be associated with activating PI3K/AKT/roTOR signal pathway,while suppressing inflammation,apoptosis and oxidative stress.

关 键 词：姜黄素 脑缺血 再灌注损伤 炎症 凋亡 氧化应激 PI3K/AKT/MTOR 

分 类 号：R743[医药卫生—神经病学与精神病学]