P2Y_2受体激动剂2-硫代-UTP及拮抗剂苏拉明对Hela细胞活性影响  

Effects of P2Y_2 Receptor Agonist 2-thio-UTP and Antagonist Suramin on the Activity of Hela Cells

作　　者：姜亚芳[1] 司马赟 黄婕[1] JIANG Ya- fang SIMA Yun HUANG Jie(Department of Clinical Laboratory, Wuzhong People＇s Hospital, Suzhou, 215128, China)

机构地区：[1]苏州市吴中人民医院检验科,江苏苏州215128

出　　处：《标记免疫分析与临床》2016年第12期1385-1389,共5页Labeled Immunoassays and Clinical Medicine

摘　　要：目的研究P2Y_2受体激动剂2-硫代-UTP及其拮抗剂苏拉明对Hela细胞活性的影响,从而在细胞水平探究P2Y_2受体在人子宫颈癌病理生理过程中的作用。方法应用CCK-8法检测不同时间点各处理组Hela细胞的活性;ELISA法分别检测细胞上清液中TNF-α和IL-6的水平;q PCR和Western blot法分别检测细胞P2Y_2mRNA和蛋白的表达变化。结果2-硫代-UTP可明显提高Hela细胞的增殖能力。且经2-硫代-UTP处理后的细胞IL-6的水平明显增加,P2Y_2mRNA和蛋白表达水平均有所提高;苏拉明可明显抑制Hela细胞增殖,经其处理后的细胞TNF-α水平有所提高,P2Y_2mRNA和蛋白表达水平无明显变化。结论 2-硫代-UTP可通过激活P2Y_2受体的活化细胞,引起细胞过表达P2Y_2受体,从而促进Hela细胞增殖。苏拉明可通过阻断P2Y_2受体及其下游活动,抑制Hela细胞增殖。以上结果表明,P2Y_2受体可能成为治疗子宫颈癌等疾病的新靶点,这将为探索子宫颈癌新型治疗手段等方面提供新思路。Objective To investigate the effect of P2Y2 receptor agonist 2-thio-UTP and antagonist suramin on the activity of in human epithelial adenocarcinoma cell line（ He La） and to explore the role of P2Y2 receptor in the pathological and physiological processes of cervical cancer in the cell level. Methods Cell counting kit-8（ CCK-8） assay was used to detect the activity of Hela cells in different time points; ELISA method was used to detect the content of TNF-α and IL-6 in the supernatants of cell; q PCR and Western blot method were used to detect the expression of P2Y2 mRNA and protein,respectively. Results 2-thio-UTP can significantly improve the proliferation of Hela cells. The content of IL-6 in the supernatants of cell increased significantly after 2-thio-UTP treatment. The expression of P2Y2 mRNA and protein were also increased. Suramincan significantly inhibit the proliferation of Hela cells,and the content of TNF-α was increased. However,the expression of P2Y2 mRNA and protein did not change significantly. Conclusion 2-thio-UTP can activate Helacells via P2Y2 receptor,and up-regulate the expression of P2Y2 receptors. As a result,it promotes the proliferation of Hela cells. Suramin could inhibit the proliferation of Hela cells by blocking the P2Y2 receptor and its downstream activities. These results suggested that P2Y2 receptor might be a newtarget for the treatment of cervical cancer and other diseases. The study provides a newidea for the exploration of newtherapeutic methods for cervical cancer.

关 键 词：P2Y2受体 2-硫代-UTP 苏拉明 HELA细胞 宫颈癌 

分 类 号：R737.33[医药卫生—肿瘤]