钙拮抗剂对大鼠脑缺血后血-脑脊液屏障通透性的影响  

Study on the effect of calcium antagonists on the permeability of blood-cerebral barrier in rats with cerebral ischemia

作　　者：许永刚[1] 黄亮[1] 张罡[1] 

机构地区：[1]湖北省仙桃市第一人民医院神经外科,湖北仙桃433000

出　　处：《临床和实验医学杂志》2017年第2期112-114,共3页Journal of Clinical and Experimental Medicine

基　　金：湖北省十一五重大科技攻关项目(编号:2013AA301A05)

摘　　要：目的观察钙拮抗剂对大鼠脑缺血后血-脑脊液屏障(BBB)通透性的影响。方法制备脑缺血再灌注(CIR)大鼠模型150只,在大鼠缺血2 h后进行再灌注。将大鼠平均分为观察组(造模后腹腔注射尼莫地平)与对照组(造模后腹腔注射生理盐水),在6 h、12 h、24 h、48 h、72 h时间点分别进行再灌注,观察每个时间点BBB通透性损伤情况与梗死灶体积百分比。结果随着时间的延长,CIR后BBB通透性与梗死灶体积百分比出现逐步上升,且在12 h、48h达到双高峰。观察组的BBB通透性及脑梗死灶体积百分比的上升较对照组较为明显(P<0.05)。结论大鼠实验结果提示,CIR能够提升BBB的通透性,增加梗死灶体积百分比,而早期使用钙拮抗剂能够加重上述情况。Objective To observe the effect of calcium antagonist on the permeability of blood- cerebral barrier in rats with cerebral ischemia.Methods 150 rats models of cerebral ischemia reperfusion（CIR） were prepared and reperfused after 2 h of ischemia.These rats were divided into observation group（intraperitoneal injection of nimodipine after modeling） and control group（intraperitoneal injection of normal saline after modeling）,reperfusion was performed at 6 h,12 h,24 h,48 h and 72 h time points respectively.The damage of permeability of blood- cerebral barrier and percentage of infarct volume had been observed at each time point.Results With the prolongation of time,the percentage of permeability of blood- cerebral barrier and volume of infarction had been gradually increased after CIR,and reached the peak at 12 h and 48 h.The permeability of blood- cerebral barrier and the percentage of volume of infarction in rats of observation group were significantly higher than those of rats in control group（P 〈0.05）.Conclusion The above results suggest that CIR can enhance the permeability of blood- cerebral barrier,increase the percentage of infarction size,and the early application of calcium antagonists can aggravate such situation.

关 键 词：大鼠 脑缺血 钙拮抗剂 血-脑脊液屏障 通透性 

分 类 号：R743[医药卫生—神经病学与精神病学]