缺氧诱导因子-1α在食管鳞癌上皮-间质转化中的作用及机制  

Effect and mechanism of hypoxia inducible factor-lol in the esophageal squamous cell carcinoma epithelialmesenchymal transition

作　　者：霍小东[1] 张洪典[2] 马钊[2] 杨明建[2] 陈传贵[2] 霍彬[1] 王海涛[1] 于振涛[2] 

机构地区：[1]天津医科大学第二医院肿瘤科,300211 [2]天津医科大学肿瘤医院食管肿瘤科国家肿瘤临床医学研究中心天津市肿瘤防治重点实验室,300060

出　　处：《中华消化外科杂志》2017年第1期83-89,共7页Chinese Journal of Digestive Surgery

基　　金：国家临床重点专科建设项目（2013-544）;天津市高等学校科技发展基金（20130121）

摘　　要：目的：检测缺氧诱导因子-1α（HIF-1α）蛋白、上皮间质转化（EMT）相关因子E钙黏素蛋白和波形蛋白在食管鳞癌组织和癌旁组织中的表达，分析3者在食管鳞癌组织中表达的相关性，探讨其与食管鳞癌患者临床病理因素及预后的关系。 方法：采用回顾性病例对照研究方法。收集2006年1月至2009年12月天津医科大学肿瘤医院收治的116例食管鳞癌患者的临床病理资料。采用免疫组织化学染色检测食 管鳞癌组织和癌旁组织中HIF-1α蛋白、E钙黏素蛋白和波形蛋白的表达。观察指标：（1）HIF-1α蛋白、E钙黏素蛋白、波形蛋白在食管鳞癌组织和癌旁组织中的表达情况。（2）HIF-1α蛋白、E钙黏素蛋白、波形蛋白在食管鳞癌组织中表达的相关性。（3）食管鳞癌患者临床病理因素（性别、年龄、肿瘤部位、肿瘤分化程度、肿瘤直径、肿瘤浸润深度、淋巴结转移、TNM分期）与HIF-1α蛋白、E钙黏素蛋白、波形蛋白阳性表达的关系。（4）食管鳞癌患者癌组织中HIF-1α蛋白、E钙黏素蛋白、波形蛋白表达与患者预后的关系。采用门诊和电话方式进行随访，了解患者生存情况。随访时间截至2014年12月。计数资料比较采 用χ2检验。相关性分析采用Spearman秩相关分析。采用KaplanMeier法计算患者生存率，Logrank检验进行生存分析。 结果：（1）HIF-1α蛋白、E钙黏素蛋白、波形蛋白在食管鳞癌组织和癌旁组织中的表达情况：食管鳞癌组织和癌旁组织中HIF-1α蛋白阳性表达率分别为50.86%（59/116）和22.22%（8/36），两者比较，差异有统计学意义（χ2=9.142，P〈0.05）；E钙黏素蛋白阳性表达率分别为25.00%（29/116）和52.78%（19/36），两者比较，差异有统计学意义（χ2=9.811，P〈0.05）；波形蛋白阳性表达率分别为32.76%（38/116）和11.11%（4/36），两者比较，差异有统计学意义（χ2=5.401，P〈0.05）Objective：To detect the expressions of hypoxia inducible factor-1α （HIF-1α） and epithelialmesenchymal transformation （EMT）related factors Ecadherin and vimentin in tumor tissues and adjacent tissues of esophageal squamous cell carcinoma （ESCC）, analyze the correlation of expressions in tumor tissues, and investigate the relationship between expressions and clinicopathological factors or prognosis. Methods：The retrospective casecontrol study was conducted. The clinicopathological data of 116 patients with ESCC who were admitted to the Cancer Hospital of Tianjin Medical University between January 2006 and December 2009 were collected. The expressions of HIF-1α, Ecadherin and vimentin in tumor tissues and adjacent tissues of ESCC were detected by immunohistochemistry （IHC）. Observation indicators： （1） the expressions of HIF-1α, Ecadherin and vimentin in tumor tissues and adjacent tissues; （2） the correlations among expressions of HIF-1α, Ecadherin and vimentin in tumor tissues; （3） the relationship between clinicopathological factors of patients with ESCC （gender, age, tumor location, tumor differentiation, tumor diameter, depth of tumor invasion, lymph node metastasis, TNM stage） and positive expressions of HIF-1α, Ecadherin and vimentin; （4） the relationship between prognosis and expressions of HIF-1α, Ecadherin and vimentin in tumor tissues. The followup using outpatient examination and telephone interview was performed to detect the survival up to December 2014. Comparison of count data was analyzed using the chisquare test. Correlation analysis was done using the Spearman rank correlation analysis. Survival rate was calculated using the KaplanMeier method and survival analysis was done using the Logrank test. Results：（1） The expressions of HIF-1α, Ecadherin and vimentin in tumor tissues and adjacent tissues： the positive expressions of HIF-1α in tumor tissues and adjacent tissues were respectively 50.86%（59/116） and 22.22%（8/36）, with a stat

关 键 词：食管肿瘤 缺氧诱导因子-1Α 上皮-间质转化 E-钙黏素 波形蛋白 

分 类 号：R735.1[医药卫生—肿瘤]