鸭输卵管壳腺部参与绿壳蛋性状形成的miRNAs富集与分析  

Enrichment and Analysis of mi RNAs Involve in the Formation of Blue Shell-Egg Trait in Duck(Anas platyrhynchos) Oviduct Shell Gland

作　　者：张涛[1] 刘贺贺[1] 罗俊[1] 胡继伟[1] 王继文[1] 甘心梦 谢艾轩 

机构地区：[1]四川农业大学畜禽遗传资源发掘与创新利用四川省重点实验室,成都611130

出　　处：《农业生物技术学报》2017年第1期133-141,共9页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(No.31301964);四川省应用基础研究计划(No.2015JY0110)

摘　　要：禽类绿壳蛋性状不仅有躲避捕食的作用,还是禽类的一个重要经济性状,由于进化和遗传背景差异,不同禽类的绿壳蛋性状形成机制不同。本研究以绿壳蛋鸭(Anas platyrhynchos)和白壳蛋鸭输卵管壳腺部组织为研究对象,基于高通量测序技术,富集和分析参与绿壳蛋性状形成的小RNA(micro RNAs,mi RNAs)及其靶基因。结果显示,在绿壳蛋鸭和白壳蛋鸭壳腺部共预测到269个mi RNAs,其中已知的mi RNAs 245个,新预测的mi RNAs 24个;相对于白壳蛋鸭,绿壳蛋鸭壳腺部存在169个差异mi RNAs,包括85个上调的mi RNAs,84个下调的mi RNAs,共预测获得4 872个靶基因。样本间mi RNAs表达量分析发现,各样品之间相关性均较高。富集基因本体(Top Gene Ontology,Top GO)分析结果显示,存在参与血红素结合的分子功能(富集基因23个,P=0.008),并且大部分均伴有电子和Fe2+的转运功能。另外,ggami R-133b、gga-mi R-193b-3p和gga-mi R-449a(log2(差异倍数(fold change,FC)>2)>1,P>0.05)预测到了参与胆绿素合成的血红素加氧酶1基因(heme oxygenase 1,HMOX1)和大量的有机物转运代谢基因。以上结果提示,部分mi RNAs可能参与了鸭绿壳蛋性状的形成调控,但可能不是影响鸭绿壳蛋形成的主要因素。研究结果为揭示家禽绿壳蛋的形成分子机制提供了理论依据,为基于基因操作技术培育绿壳蛋禽类提供了基础资料。Blue shell trait is not only a protective coloration that can avoid predation, but also is one of the important economic traits of poultry. Due to the differences of evolutionary and genetic backgrounds, the formation mechanism of blue shell egg are divergent in different poultries. By using the high-throughput sequencing, the oviduct shell gland of blue egg shell ducks and white egg shell ducks were studied to enrich and analyze micro RNAs（mi RNAs） and its target genes involved in formation of blue shell egg traits. The analysis result of sequencing data quality showed the GC content of each sample was not less than 42.60%,and the proportion of clean reads that was ≥ 30 bases（Q30） was not less than 89.02%. Compared with the chicken（Gallus gallus） genome sequence（Gallusgallus-4.0, GCF000002315.3） using the mi RDeep2software, all 269 mi RNAs were screened in the shell gland of blue shelled ducks and white shelled ducks,including known 245 mi RNAs and new 24 mi RNAs. Based on the expression of mi RNAs in each sample, the correlation analysis was performed between the all of each samples. The result showed that the correlation coefficient were higher between all of the shell gland samples in blue and white shelled ducks（r〉0.99）,which suggested that mi RNAs expression profiles might be similar in the shelled gland of blue and white shelled ducks. Compared with white shelled ducks, the shell gland of blue shelled ducks had 169 differently expressed mi RNAs, including 85 up-regulated and 84 down-regulated, and total 4 872 target genes were obtained, but the mi RNAs were not significant difference（P〉0.05）. The Top Gene Ontology（Top GO）analysis of target genes showed that molecules function including heme binding（count=23, P=0.008） were existed, and most of them were accompanied by function of electron and Fe2 ＋transport. In addition, the analysis of differently expressed mi RNAs found that biliverdin synthetic gene — heme oxygenase 1（HMOX1）and a large number of organic matte

关 键 词：绿壳蛋 鸭壳腺部 mi RNAS 高通量测序技术 胆绿素 

分 类 号：S834.83[农业科学—畜牧学]