胆结石患者血清和胆汁幽门螺杆菌相关蛋白免疫印迹检测  

The Analysis of Associated Protein of Helicobacter Pylori in Serum and Gallbladder Bile of Gallstone Patients

作　　者：郭吕[1] 唐俐[1] 郭新瑛[1] 朱景元[1] 王学东[1] 张月林[1] 陶卫东[1] 胡兵[1] 倪泉兴[2] 

机构地区：[1]江苏昆山中医院,南京215300 [2]复旦大学华山医院外科

出　　处：《临床消化病杂志》2002年第4期168-170,共3页Chinese Journal of Clinical Gastroenterology

基　　金：江苏省自然科学基金(BS99068)

摘　　要：目的:研究幽门螺杆菌(HP)感染与胆石形成的关系。方法:采用聚合酶链免疫吸附法(ELISA)对胆结石患者的血清及胆汁免疫球蛋白IgG、IgA、IgM标本进行检测,对血清学及胆汁IgG均阳性患者的胆汁,采用免疫印迹法(Western blot)进行HP感染相关蛋白检测。结果:胆结石组50例患者中血清学及胆汁IgG均阳性者29例(58.0％)。另取非胆结石组13例作为对照。胆结石组与非胆结石组血清及胆汁HP免疫印迹检测,均可检出4种主要HP感染相关蛋白,但胆结石组血清及胆汁中Ure A检出率较非胆结石组高。结论:胆汁中存在多种HP感染相关蛋白,并可能参与胆结石的形成。Purpose: To investigate whether helicobacter pylori (HP) infection was associated with the formation of gallstone. Methods: In 50 patients, the gallbladder was removed for gallstone disease with their serum and bile examined for HP immunoglobulin G by enzyme-linked immunosorbent assays (ELISA). In all the immunoglobulin G positive cases, HP associated protein of the serum and bile samples was examined by Western blotting technique. Results: The immunoglobulin G were positive in 29 cases of all serum and bile samples(58.0%) in gallstone group. Thirteen cases in non-gallstone group were used as control. In both group all contained HP anti-Cag A, Vac A, Ure B and Ure A antibodies in serum and bile samples, but positive rates of the HP-Ure A in gallstone group were higher than non-gallstone group. Conclusion: It is suggested that presence of associated protein of HP infection in most bile samples, may be a participate factor to increase the risk of gallstone formation.

关 键 词：幽门螺杆菌 免疫球蛋白 免疫印迹 胆石症 

分 类 号：R575.6[医药卫生—消化系统]