鹅肥肝形成中补体受体1基因的表达和调控研究  

Study on the Expression and Regulation of Goose Complement Receptor 1 Gene during Goose Fatty Liver Formation

作　　者：刘龙[1] 王倩[1] 许程[1] 赵星[1] 李富原 赵盼[1] 耿拓宇[1] 龚道清[1] LIU Long WANG Qian XU Cheng ZHAO Xing LI Fuyuan ZHAO Pan GENG Tuoyu GONG Daoqing(College of Animal Science and Technology,Yangzhou University,Yangzhou,Jiangsu 22500)

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《中国家禽》2016年第24期5-10,共6页China Poultry

基　　金：国家自然科学基金项目(31072021;31372298;31501945);江苏省农业自主创新项目(CX(12)2033);扬州大学大学生学术科技创新基金项目

摘　　要：试验旨在探讨鹅补体受体1(CR1)基因在鹅肥肝形成中的表达和调控机制。通过荧光定量PCR验证CR1在填饲19 d鹅肝脏中的表达情况,通过体外细胞(鹅原代肝细胞)试验探索脂肪肝相关因子(葡萄糖、胰岛素、油酸和棕榈酸)对CR1的诱导作用,同时通过分析CR1基因的上游序列预测其转录调控因子并通过药物处理进行验证。结果发现,CR1基因在填饲19 d肥肝中的表达量显著高于对照组;25 mmol/L葡萄糖以及各处理浓度的油酸(0.125、0.25和0.5 mmol/L)均可导致鹅原代肝细胞中CR1表达水平的显著上调,胰岛素对CR1的表达水平没有显著影响,而0.5 mmol/L棕榈酸则对CR1在鹅原代肝细胞中的表达有一定的抑制作用;序列分析发现,鹅CR1上游序列含有肝细胞核因子1(HNF1)的结合位点,且使用HNF1的活性调控剂(鹅去氧胆酸)处理鹅原代肝细胞后,鹅CR1基因的表达量显著上调。综上,本研究证实CR1基因在鹅肥肝形成过程中表达水平的显著上调,并对其可能的调控机制进行初步探索,为深入研究补体系统在鹅肥肝形成中的作用和机制奠定了基础。The aim of this study is to investigate the expression and regulation of goose complement receptor 1-CR1 gene in the formation of goose fatty liver. The CR1 expression in livers of geese after 19 days＇ overfeeding was validated by qRT-PCR,the effect of fatty liver-related factors （glucose,insulin,oleate and palmitate）on CR1 expression in vitro （goose primary hepatocytes）was explored and the upstream sequence of CR1 were analyzed,then its transcription factor （TF were predicted）,and the predicted TF were validated through chemical treatment. The results indicated that CR1 expression was significantly increased in goose fatty liver after 19 days＇ overfeeding, and 25 mmol/L glucose as well as different concentrations of oleate （0.125,0.25 and 0.5 mmol/L） could induce the expression of CR1 in goose primary hepatocytes. However,insulin seemed to have no effect on CR1 expression and 0.5 mmol/L palmitate even suppressed the expression of CR1. By sequence analysis,HNF1 was predicted as the transcription factor of goose CR1.Then goose primary hepatocytes were treated with chenodeoxycholic acid （which can regulate the expression of HNF1） and found that CR1 expression was significantly induced. In conclusion, it was confirmed that the up-regulation of CR1 expression in goose fatty liver, and primarily explored its regulation mechanism. This study laid a foundation for further investigation of complement system in goose fatty liver formation.

关 键 词：鹅 补体受体1 肥肝 鹅原代肝细胞 

分 类 号：S835[农业科学—畜牧学]