肠易激综合征患者炎性细胞因子的表达与酪酪肽、密封蛋白-1的相关性  

Expression of inflammatory cytokines and its correlation with peptide YY and claudin-1 in patients with irritable bowel syndrome

作　　者：王燕[1] 刘欣[1] 黄小平[1] 李永[1] 罗金燕[1] 

机构地区：[1]西安交通大学第二附属医院消化科,710004

出　　处：《中华消化杂志》2016年第12期805-810,共6页Chinese Journal of Digestion

基　　金：国家自然科学基金（81100258）;博福一益普生腹泻基金课题（IDF-2012-02）

摘　　要：目的通过检测IBS患者血清和结肠黏膜中炎性细胞因子与酪酪肽、密封蛋白1的表达及其相关性，探讨IBS的发病机制。方法选取2013年4月至2015年4月IBS门诊患者50例和健康对照者20名，采用ELISA法检测其血清酪酪肽、IL-10、TNF-α和密封蛋白-1水平；采用免疫组织化学法检测结肠黏膜中酪酪肽、IL-10、TNF-α和密封蛋白-1的蛋白表达水平。统计学分析采用单因素方差分析、最小显著差异（LSD）法、卡方检验和Pearson相关性分析。结果50例IBS患者中，腹泻型IBS组27例，便秘型IBS组23例。腹泻型IBS组血清酪酪肽水平及其结肠黏膜阳性表达率均高于健康对照组[（16.28±2.75） ng/L比（10.12±1.55） ng/L，66.7%（18/27）比30.0%（6/20）]，差异均有统计学意义（LSD-t＝10.19，χ2＝6.182；P均〈0.05）。腹泻型IBS组血清IL-10水平及其结肠黏膜表达率均低于健康对照组[（2.95±0.24） ng/L比（3.58±0.35） ng/L，22.2%（6/27）比50.0%（10/20）]，差异均有统计学意义（LSD-t＝4.52，χ2＝3.948；P均〈0.05），血清TNF-α水平及其结肠黏膜表达率均高于健康对照组[（8.73±0.41） ng/L比（7.73±0.51） ng/L，66.7%（18/27）比30.0%（6/20）]，差异均有统计学意义（LSD-t＝8.43，χ2＝6.182；P均〈0.05）。便秘型IBS组与健康对照组血清酪酪肽、IL-10、TNF-α水平[（10.24±1.34） ng/L比（10.12±1.55） ng/L，（3.43±0.71） ng/L比（3.58±0.35） ng/L，（7.81±0.26） ng/L比（7.73±0.51） ng/L ]及其结肠黏膜阳性表达率[39.1%（9/23）比30.0%（6/20），56.5%（13/23）比50.0%（10/20），34.8%（8/23）比30.0%（6/20）]比较差异均无统计学意义（P均〉0.05）。腹泻型IBS组血清密封蛋白-1水平及其结肠黏膜表达阳性率均低于健康对照组[（94.44±6.61） ng/L比（103.64±5.47） ng/L，11.1%（3/27）比40.0%（8/20）]，差异均有统计学意义（LSD-t＝5.76，χ2＝5.349；P均〈0.05）；便秘型IBS组�ObjectiveTo explore the pathogenesis of irritable bowel syndrome （IBS） by detecting serum levels and the colonic mucosa expression of inflammatory cytokines, peptide YY （PYY）, and claudin-1, and to analyze their correlation.MethodsFrom April 2013 to April 2015, 50 outpatients with IBS and 20 healthy controls were selected. Serum levels of PYY, interleukin （IL）-10, tumor necrosis factor （TNF）-α and claudin-1 were detected by enzyme-linked immunosorbent assay （ELISA）. The expression of IL-10, TNF-α, PYY and claudin-1 in colonic mucosa was determined by immunohistochemistry. Single factor analysis of variance, least significant difference （LSD） method, chi-square test, and Pearson correlation analysis were performed for statistical analysis.ResultsAmong the 50 patients with IBS, 27 cases were diarrhea-type irritable bowel syndrome （D-IBS）, and 23 cases were constipated-type irritable bowel syndrome （C-IBS）. The serum level and the positive expression rate of PYY in colonic mucosa of D-IBS group were significantly higher than those of healthy control group （（16.28±2.75） ng/L vs （10.12±1.55） ng/L; 66.7%（18/27） vs 30.0%（6/20））, and the differences were statistically significant （LSD-t=10.19, χ2=6.182, both P〈0.05）. The serum level and the positive expression rate of IL-10 in colonic mucosa of D-IBS group were both significantly lower than those of healthy control group （（2.95±0.24） ng/L vs （3.58±0.35） ng/L; 22.2%（6/27） vs 50.0%（10/20））, and the differences were statistically significant （LSD-t=4.52, χ2=3.948, both P〈0.05）. The serum level and the positive expression rate of TNF-α in colonic mucosa of D-IBS group were both significantly higher than those of healthy control group （（8.73±0.41） ng/L vs （7.73±0.51） ng/L; 66.7%（18/27） vs 30.0%（6/20））, and the differences were statistically significant （LSD-t=8.43, χ2=6.182, both P〈0.05）. There was no statistically significant difference between C-IBS grou

关 键 词：肠易激综合征 肿瘤坏死因子α 白细胞介素10 肽YY 密封蛋白-1 

分 类 号：R574.4[医药卫生—消化系统]