β-拉帕醌通过下调过氧化物酶V诱导SW480结肠癌细胞凋亡  

β-lapachone Induced the Apoptosis of SW480 Colorectal Cancer Cell through Down-Regulation of the Peroxiredoxin V Expression

作　　者：刘悦[1] 焦冰洋 于楠楠[1] 王闯[1] 韩英浩[1] 金成浩[1] 孙虎男[1] Liu Yue Jiao Bingyang Yu Nannan Wang Chuang Han Yinghao Jin Chenghao Sun Hunan(College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 1633419, China)

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,大庆163319

出　　处：《中国细胞生物学学报》2016年第12期1487-1493,共7页Chinese Journal of Cell Biology

基　　金：黑龙江八一农垦大学大学生创新创业训练项目(批准号:XC2015049);黑龙江省大学生创新创业训练项目(批准号:201610223035)资助的课题~~

摘　　要：过氧化物酶V(peroxiredoxin V,Prx V)是过氧化物酶(peroxiredoxin)家族中的一员,广泛存在于线粒体、溶酶体及细胞质中,具有有效地清除细胞内一氧化氮(nitric oxide,NO)和活性氧(reactive oxygen species,ROS)的功能。该文阐明β-拉帕醌(β-lapachone)引起SW480结肠癌细胞凋亡过程中Prx V的作用及其机制。该研究利用β-拉帕醌处理SW480细胞系,通过3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐[3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide,MTT]法、荧光显微镜照相、流式细胞术、蛋白质免疫印迹分析等方法检测细胞存活率、细胞内ROS水平、细胞凋亡情况以及凋亡相关蛋白质水平的变化。结果表明,β-拉帕醌通过抑制Prx V水平,致使细胞内ROS水平升高,激活线粒体依赖性凋亡途径,导致SW480结肠癌细胞凋亡。研究结果初步揭示了β-拉帕醌诱发SW480结肠癌细胞发生凋亡的机制,为结肠癌的治疗提供了新思路。Peroxiredoxin V （Prx V） is a thioredoxin peroxidase included in peroxiredoxins family, which is highly expressed in mitochondria, lysosomes and cytosols, and exhibits as scavengers for nitric oxide （NO） and reactive oxygen species （ROS）. This study is aimed to explore the regulatory effect of Prx V on β-lapachone induced apoptosis of SW480 colorectal cell. To examine the SW480 cell viability, cellular ROS levels, apoptosis and the proteins levels, the MTT assay, fluorescence microscopy, flow cytometry and Western blot assay were performed. The results showed that β-lapachone treatments could decrease the Prx V protein level, which in turn increased the cellular ROS levels, then eventually activated the mitochondria signaling, and resulted in inducing the SW480 cell apoptosis. Our findings present a new therapeutic strategy for colorectal cancer.

关 键 词：过氧化物酶V 活性氧 细胞凋亡 β-拉帕醌 

分 类 号：R735.35[医药卫生—肿瘤]