miR103通过靶向PDCD10逆转顺铂诱导的前列腺癌细胞耐药  

miR103 reversed the resistance of cisplatin in prostatic cancer cells by targeting PDCD10

作　　者：付晓亮[1] 杨增悦[1] 舒涛[1] 王东[1] 

机构地区：[1]第四军医大学附属唐都医院泌尿外科,陕西西安710038

出　　处：《现代肿瘤医学》2017年第4期511-515,共5页Journal of Modern Oncology

基　　金：中国人民解放军第四军医大学校基金(编号:2016XD284)

摘　　要：目的:探讨miR103在前列腺癌细胞耐药中的作用及机制。方法:RT-PCR检测miR103在人前列腺癌细胞系PC-3、LNCap、22Rv1和DU145以及顺铂耐药细胞株PC-3/DDP、LNCap/DDP、22Rv1/DDP和DU145/DDP中的表达。以PC-3/DDP细胞系为研究对象,采用miR103 mimic或inhibitor转染细胞,流式细胞仪检测细胞凋亡,Western blotting和RT-PCR检测PDCD10的表达。采用PDCD10过表达载体转染过表达miR103的细胞,流式细胞仪检测细胞凋亡,Western blotting和RT-PCR检测细胞P糖蛋白(P-gp)的表达。结果:与亲本细胞系比较,miR103在顺铂耐药细胞系中低表达。上调miR103,顺铂诱导的细胞凋亡显著增加;下调miR103,顺铂诱导的细胞凋亡减少,但不具有显著性。过表达miR103,PDCD10的表达显著减少。转染PDCD10过表达载体显著减少miR103 mimic诱导的PC-3/DDP细胞凋亡,逆转了miR103 mimic对P-gp表达的抑制作用。结论:miR103通过靶向PDCD10抑制P-gp的表达逆转前列腺癌细胞耐药。Objective： To explore the effect of miR103 on the resistance of cisplatin in prostatic cancer cells and its underlying mechanism. Methods： The expression of miR103 in human prostatic cancer PC- 3,LNCap,22 Rv1,DU145 cell lines and cisplatin- resistant PC- 3 / DDP,LNCap / DDP,22 Rv1 / DDP,DU145 / DDP cell lines were determined by RT- PCR. The PC- 3 / DDP cell line was chosen as experimental object. miR103 mimic or inhibitor was transfected into the cells,and cell apoptosis was detected by flow cytometer. The miR103 mimic or PDCD10 overexpression vector was transfected into the cells respectively and the expression levels of PDCD10 were determined by Western blotting and RT- PCR. PDCD10 overexpression vectors were transfected into miR103 mimic transfected cells,and cell apoptosis was detected by flow cytometer. The expression of P- gp was determined in miR103 overexpressing and PDCD10 overexpressing cells by Western blotting and RT- PCR. Results： The expression level of miR103 was significantly decreased in cisplatin- resistant cell lines compared with parent cell lines. Cell apoptosis rate was significantly increased in miR103 mimic transfected PC- 3 / DDP cells,but decreased slightly in miR103 inhibitor transfected cells. The expression of PDCD10 was significantly decreased in miR103 mimic transfected PC- 3 /DDP cells. Overexpression of PDCD10 significantly reduced cell apoptosis in miR103 mimic transfected PC- 3 / DDP cell,and neutralized the inhibition of P- gp expression induced by miR103 mimic. Conclusion： miR103 can reverse drug resistance of prostatic cancer cells though inhibiting the expression of P- gp by targeting PDCD10.

关 键 词：miR103 PDCD10 P糖蛋白 前列腺癌 

分 类 号：R737.25[医药卫生—肿瘤]