藤黄酸对人肾癌786-O细胞增殖和凋亡的影响  

Effect of Gambogic acid on proliferation and apoptosis in 786-O cells

作　　者：王静[1] 刘涛[1] 郭春华[1] 张雅雅[1] 刘蕾[1] 万幼峰[1] 

机构地区：[1]中国人民解放军第一七四医院,厦门大学附属成功医院肿瘤/血液科,福建厦门361001

出　　处：《现代肿瘤医学》2017年第4期531-535,共5页Journal of Modern Oncology

摘　　要：目的:观察藤黄酸(Gambogic acid)对人肾癌786-O细胞增殖和凋亡的影响,并初步探讨其可能的作用机制。方法:MTT法检测藤黄酸对786-O细胞的增殖抑制作用。流式细胞术检测藤黄酸对786-O细胞凋亡的影响。Western blotting检测藤黄酸对786-O细胞内凋亡相关蛋白Bcl-2、Bax及NF-κB通路相关蛋白p65、IκB表达的影响。荧光显微镜观察藤黄酸对NF-κB活性的影响。结果:藤黄酸对786-O细胞的增殖抑制率具有显著的剂量依赖性,在作用48h后其IC50值为(3.4±0.3)μmol/L。细胞凋亡实验发现,藤黄酸能有效的诱导786-O细胞凋亡,随药物浓度增加细胞凋亡比例逐渐增大,当药物浓度达到4.0μmol/L时凋亡率为(68.1±3.6)%。Western blotting发现藤黄酸能引起Bax蛋白的上调和Bcl-2蛋白的降解,与此同时能抵抗TNF-α诱导导致的IκB降解;荧光显微成像则进一步发现藤黄酸能阻止NF-κB入核。结论:实验表明,藤黄酸能诱导786-O细胞凋亡,主要机制可能是通过其对NF-κB的信号通路的抑制实现的。Objective： To observe the effect of Gambogic acid on proliferation and apoptosis of human renal cell carcinoma 786- O cells,and its possible mechanism. Methods： MTT assay and flow cytometry technology were used to detect the function of Gambogic acid on proliferation and apoptosis of 786-O cells respectively. After treating with Gambogic acid,expression level of Bcl- 2,Bax,p65,and IκB in 786- O cells were determined by Western blotting assay,and the NF- κB activity was monitored by fluorescence microscopy. Results： Gambogic acid showed an significant activity of proliferation inhibition on 786- O cells by dose- dependence after 48 h,and the IC50 value was（ 3. 4± 0. 3） μmol / L. Accompany with proliferation inhibition,Gambogic acid was also able to induce the apoptosis of 786- O cells effectively by dose- dependence,and the apoptosis rate reached at（ 68. 1 ± 3. 6） % when the concentration was 4. 0 μmol / L. Western blotting result found that Gambogic acid can down- regulate the expression levels of Bcl-2 protein and up- regulate the expression levels of Bax protein in 786- O cells respectively. Meanwhile,it showed an resistant to IκB degradation which induced by TNF- α- IκB. Fluorescence microscopy assay further confirmed that Gambogic acid was able to prevent NF- κB translocating into the nucleus. Conclusion： Gambogic acid could induce the apoptosis of 786- O cells,and the activity inhibition on NF- κB signaling pathways may contribute to the apoptosis mechanism.

关 键 词：藤黄酸 786-O细胞 凋亡 

分 类 号：R737.12[医药卫生—肿瘤]