低氧对再生医学中间充质干细胞培养的研究进展  

The advance of hypoxia on mesenchymal stem cell in regenerative medicine

作　　者：张姝婷[1] 韩潇[1] 赵强[1] 白海[1] 

机构地区：[1]兰州军区兰州总医院血液科,甘肃兰州730050

出　　处：《现代肿瘤医学》2017年第4期661-665,共5页Journal of Modern Oncology

基　　金：甘肃省科技重大专项(编号:1102FKDA005)

摘　　要：由于间充质干细胞(MSCs)具有自我更新、免疫抑制能力和多系分化潜能等特征,因此被认为是干细胞再生治疗中一个重要资源。然而,体外扩增期间的低生产动力学、早期衰老、基因不稳定性和低移植率是MSCs在再生治疗中的主要不利因素。细胞间及细胞内大量的信号通路在其壁龛中控制着MSCs的生长、倍增和分化。通常,干细胞培养的实验条件为20%的环境O_2浓度,而壁龛中通常为2%~9%O_2。O_2通过调节乏氧诱导因子-1(HIF-1)来介导不同基因的表达,从而对维持干细胞增殖和分化具有重要作用。本文主要描述和对比常氧(20%O_2)和低氧(2%~9%O_2)对MSCs的生物学影响。并总结了在体外培养扩增中,低氧环境显著提高MSCs的生长动力学、遗传稳定性和趋化因子受体的表达,最终提高MSCs在再生治疗中的效应。Because mesenchymal stem cells（ MSCs） have self- renewing,immunosuppressive property,multi- lineage differentiation potential,and so on. Therefore they are considered as important resource of stem cells for regenerative therapy. However,poor production kinetics,early senescence,genetic instability,and poor transplantation rates in vitro expansion period are the main negative factors in MSC- based regenerative therapy. A large number of inter-and intra- cellular signaling pathways control the growth,multiplication,and differentiation of MSCs in their niche. Usually,the stem cells culture of experimental conditions was 20 % ambient O2 concentration,while their niche is 2 % -9 % O2. O2 mediate expression of various genes through regulating hypoxia- inducible factor- 1（ HIF- 1）,and play an important role in maintaining stem cell of proliferation and differentiation. This paper describes and contrasts the role of normoxic（ 20 % O2） and hypoxia（ 2% - 9% O2） on the biological effects of MSCs. Finally,we concluded that hypoxic environment can significantly improve the growth kinetics,genetic stability,and expression of chemokine receptors during in vitro culture and expansion,and ultimately improve the effects of MSCs in regenerative therapy.

关 键 词：间充质干细胞 低氧 乏氧诱导因子-1 

分 类 号：R730.2[医药卫生—肿瘤]