健康产品中非法添加利巴韦林的分散固相萃取-高效液相色谱筛查及质谱确证  

Ribavirin in Health Products by Dispersive Solid-Phase Extraction HPLC Screening and LC-MS/MS Verification Method

作　　者：罗伟[1] 李旸华[1] 林秋凤[1] 

机构地区：[1]东莞市食品药品检验所,广东东莞523109

出　　处：《儿科药学杂志》2017年第1期39-42,共4页Journal of Pediatric Pharmacy

基　　金：广东省药检系统科研项目;编号XJ201201

摘　　要：目的:建立健康产品(食品、保健品、药品)中非法添加利巴韦林的快速筛查及确证方法。方法:采用分散固相萃取-高效液相色谱(HPLC)快速筛查抗病毒药品。样品(胶囊、冲剂、片剂、口服液均可)提取后,加入固相萃取填料净化,采用Phenomenex强阳离子氢型交换色谱柱(300 mm×7.8 mm),以水(稀硫酸调至p H 2.5±0.1)为流动相,流速0.5 m L/min,柱温60℃,检测波长207 nm。阳性样品采用液相色谱-串联质谱(LC-MS/MS)进行确证。结果:利巴韦林在0.01~0.30 mg/m L浓度范围内线性关系良好(r=0.999 7),加样回收率为100.9%,RSD为0.8%。结论:该方法可以简便、准确、灵敏地测定健康产品中非法添加的利巴韦林。Objective： To establish a rapid screening and confirmation method of ribavirin in antivirus health products. Methods： The samples were screened by dispersive solid-phase extraction HPLC,and extracted and purity with solid phase extraction purification.HPLC was used with a Phenomenex Rezex ROA-Organic Acid H＋column（ 300 mm× 7. 8 mm） using water（ adjusted to p H 2. 5± 0. 1with sulfuric acid） as the mobile phase. The detection wavelength was 207 nm and flow rate was 0. 5 m L / min. The column temperature was 60 ℃. The positive samples were verified by LC-MS / MS. Results： The ribavirin curve was linear within a range of 0. 01 to 0. 30 mg / mL. The average recovery was 100. 9% with RSD was 0. 8%. Conclusion： This method is convenient,accurate and sensitive,it could be used to the determination of ribavirin in health products.

关 键 词：利巴韦林 分散固相萃取 高效液相色谱 质谱 

分 类 号：R917[医药卫生—药物分析学]