海马线粒体通透性转换孔在深低温停循环大鼠模型中的开闭情况研究  

Opening and closing of mitochondrial permeability transition pore in deep hypothermic circulatory arrest in rat models

作　　者：孔繁熙 张晶[2] 朱耀斌[3] 李志强[4] 范祥明[3] 刘东海[5] 张伟华[5] 廖秋明[6] 杨尧[3] 刘扬[3] 乔晨晖[5] 

机构地区：[1]内蒙古自治区人民医院胸心外科,呼和浩特010017 [2]新疆维吾尔自治区人民医院心外科,乌鲁木齐830001 [3]首都医科大学附属北京安贞医院小儿心脏中心,北京100029 [4]首都医科大学附属北京儿童医院小儿心脏中心,北京100037 [5]郑州大学第一附属医院心外科,郑州450052 [6]瑞典隆德大学医院心胸外科,隆德se22185

出　　处：《中华实用诊断与治疗杂志》2016年第12期1166-1169,共4页Journal of Chinese Practical Diagnosis and Therapy

基　　金：国家自然科学基金(81371443);国家自然科学基金(81400305);北京市自然科学基金(7122056);北京市自然科学基金(7142049);北京市自然科学基金(7142137);北京市自然科学基金(7152045);新疆维吾尔自治区自然科学基金(2014211A063);首都医科大学基础-临床科研合作基金(13JL26);北京市优秀人才培养资助(2014000021469G233);北京市卫生局高层次人才培养资助(2014-3-043);北京市卫生局高层次人才培养资助(2015-3-048);北京市卫生局高层次人才培养资助(2015-3-051);北京市属医院科研培育项目(PX2016046)

摘　　要：目的探讨深低温停循环大鼠模型海马线粒体通透性转换孔的开闭情况,及深低温对脑保护的作用机制。方法21只大鼠随机分为深低温停循环组、常温停循环组和对照组,每组7只。深低温停循环组大鼠体温降至20℃以下体外循环10min,维持深低温条件下停循环10min;常温停循环组大鼠常温体外循环10min,停循环10min;对照组大鼠常温体外循环20min,不降温不停循环。采用酯化钙黄绿素钴荧光技术检测3组大鼠海马组织荧光染色情况,采用Western blot法检测海马线粒体细胞色素C表达情况。结果对照组海马组织荧光亮度最强,常温停循环组荧光亮度最弱,深低温停循环组荧光亮度介于对照组和常温停循环组之间;深低温停循环组和对照组大鼠海马组织荧光强度值(1 387.9±19.3、1 774.6±48.3)和线粒体细胞色素C灰度值比(16.82±1.47、23.54±1.86)高于常温停循环组(1 125.6±37.4、12.31±1.43)(P<0.05)。结论停循环能使大鼠海马线粒体通透性转换孔开放增加,深低温能降低海马线粒体通透性转换孔的开放数量,具有脑保护作用。Objective To study the opening and closing of the mitochondrial permeability transition pore in deep hypothermic circulatory arrest in rat models,and to explore the mechanism of brain protection of deep hypothermia.Methods Twenty-one rats were randomly divided into deep hypothermic circulatory arrest group,room-temperature circulatory arrest group and control group,with 7 rats in each group.The body temperature dropped below 20 ℃,and the rats received cardiopulmonary bypass（CPB）for 10 min followed by circulatory arrest for 10 min in deep hypothermic circulatory arrest group.The rats at room temperature received CPB for 10 min,followed by circulatory arrest for 10 min in room-temperature circulatory arrest group.The rats received CPB for 20 min at room temperature without circulatory arrest in control group.The hippocampal tissue fluorescence staining was detected by esterification cobalt calcein fluorescence,and hippocampal cytochrome C expression was detect by Western blot method.Results The hippocampal tissue fluorescence intensity was the strongest in control group,followed by room-temperature circulatory arrest group and deep hypothermic circulatory arrest group in turns.The hippocampal tissue fluorescence intensity values（1 387.9±19.3,1 774.6±48.3）and the gray values of mitochondrial cytochrome C（16.82±1.47,23.54±1.86）in deep hypothermic circulatory arrest group and control group were significantly higher than those in room-temperaturecirculatory arrest group（1 125.6±37.4,12.31±1.43）（P〈0.05）.Conclusion Circulatory arrest can increase the opening of hippocampus permeability transition pore,and deep hypothermia can reduce the closing times of hippocampus mitochondrial permeability transition pore with neuroprotective effect.

关 键 词：停循环 深低温 通透性转换孔 大鼠 线粒体 

分 类 号：R-332[医药卫生]