抗胶质瘤Ⅰ号方对脑胶质瘤细胞侵袭性的影响  

The Effect of Resistance to GliomaⅠPrescriptions on the Invasion of Glioma Cells

作　　者：刘春玲 唐明科 秦丽娟 赵喜庆 

机构地区：[1]内蒙古赤峰市第二医院,内蒙古赤峰024000 [2]华北理工大学

出　　处：《中国医学创新》2016年第36期14-17,共4页Medical Innovation of China

基　　金：国家自然科学基金项目(81101912);河北省中医药管理局中医药类科研项目(2014195);河北省高等学校科学技术研究项目(ZD20160082)

摘　　要：目的:探讨抗胶质瘤Ⅰ号对胶质瘤细胞侵袭性降低的作用及其与PI3K/PKB信号传导通路之间的关系。方法:MTT法检测抗胶质瘤Ⅰ号处理培养的C6胶质瘤细胞的抑制率,免疫印迹法检测肿瘤细胞内PI3K和PKB表达水平,最后,利用结晶紫染色法检测Transwell下室面的胶质瘤细胞数。结果:抗胶质瘤Ⅰ号可以抑制PI3K和PKB蛋白表达水平,抗胶质瘤Ⅰ号作用0.5 h时PI3K蛋白表达水平最低为(25.1±3.1),作用1 h时PKB蛋白表达水平最低为(25.1±3.8),与对照组的(99.1±1.6)、(91.2±3.5)比较,差异均有统计学意义(P<0.05)。而抗胶质瘤Ⅰ号作用3 h时对胶质瘤细胞的抑制程度最大,抑制率为(91.2±5.7)%,与对照组的(68.0±5.2)%比较,差异有统计学意义(P<0.01)。且此时Transwell下室面的细胞数最少,为(1.4±0.4)×106个,与对照组的(35.6±2.7)×106个比较,差异有统计学意义(P<0.05)。结论:抗胶质瘤Ⅰ号可能是通过抑制PI3K/PKB而抑制胶质瘤细胞的增殖,从而引起胶质瘤细胞侵袭性的降低。Objective： To investigate the effect of Resistance to Glioma I Prescriptions on reducing the invasiveness of glioma cells, and the relationship between Resistance to Glioma I Prescriptions and PI3K/PKB signal transduction pathway.Method ： MTT method was used to detect cell inhibition rate of C6 glioma cells which was treated by the Resistance to Glioma I Prescriptions, the expression level of PI3K and PKB in tumor cells was detected by Western blot, in the end, the number of glioma cells in Transwell was detected by crystal violet staining method.Result： Resistance to Glioma I Prescriptions can inhibit PI3K and PKB protein expression level, the expression level of PI3K protein was the lowest （ 25.1 ± 3.1 ） when the effect of Resistance to Glioma I was 0.5 h, and the lowest level of PKB protein expression was （ 25.1 ±3.8 ） at the effect of Resistance to glioma I was 1 h, compared with the control group （ 99.1 ± 1.6 ）, （ 91.2 ±3.5 ）, the differences were statistically significant （ P〈0.05 ） .While the largest inhibition rate appear at 3 h after Resistance to Glioma I Prescriptions, inhibition rate was （ 91.2 ±5.7 ） %, compared with （ 68.0± 5.2 ） % in control group, the difference was statistically significant （ P〈0.01 ） .At the same time, the glioma cell number on transwell chamber surface was the least （ 1.4 ± 0.4 ） ×l06, compared with （ 35.6 ± 2.7 ） × 106 in control group, the difference was statistically significant （ P〈0.05 ） . Conchmion： Resistance to Glioma I Prescriptions may is through the inhibition of PI3K/PKB signal transduction pathway and inhibit the proliferation of glioma cells, thereby reducing the invasiveness of glioma ceils.

关 键 词：胶质瘤 抗胶质瘤Ⅰ号 PI3K/PKB信号传导通路 

分 类 号：R739.41[医药卫生—肿瘤]