联合应用Wee1抑制剂和阿霉素对急性白血病细胞株HL-60作用的研究  

The Effect of Wee1 Inhibitor Combined with Adriamycin on HL-60 in Acute Leukemia Cell Line

作　　者：张西凯[1] 于广晴[1] 倪蕾[1] 王为光[1] 李柏林[1] 

机构地区：[1]佳木斯大学附属第一医院,黑龙江佳木斯154003

出　　处：《中国医学创新》2016年第36期18-20,共3页Medical Innovation of China

基　　金：佳木斯大学研究生科技创新项目(YM2016_025)

摘　　要：目的:通过联合应用PD0166285和阿霉素,观察其对急性白血病细胞HL-60的作用,探讨其可能的作用机制。方法:通过MTT法检测药物对HL-60的生长抑制作用,流式细胞术检测其凋亡作用,Western bolt检测凋亡相关蛋白caspase-3和PARP的表达情况。结果:PD0166285联合阿霉素对HL-60细胞的抑制作用和促凋亡作用明显增强,同时凋亡相关蛋白caspase-3和PARP的剪切增加。结论:PD0166285能够增强阿霉素对HL-60细胞的生长抑制并促进其凋亡,可能通过caspase依赖性凋亡途径发挥作用。Objective： Through the combined use of PD0166285 and adriamyein, to observe its effect on acute leukemia cells HL-60, and to explore its possible mechanism.Method： MTT method was used to detect the inhibitory effect of drugs on the growth of HL-60, flow eytometry was used to detect the apoptosis, Western bolt was used to detect the expression of apoptosis related protein caspase-3 and PARP.Result： The inhibitory effect and apoptosis of PD0166285 combined with adriamyein on HL-60 cells was significantly enhanced, and the shear of apoptosis related protein easpase-3 and PARP increased.Conclusion： PD0166285 can enhance the growth inhibition and apoptosis of HL-60 cells, which may play a role in caspase dependent apoptosis pathway.

关 键 词：PD0166285 ADM HL-60 凋亡 

分 类 号：R733.71[医药卫生—肿瘤]