利用PCR—SSP技术检测TAP2等位基因多态性  

Using PCR-SSP technique to detect the polymorphism of TAP2 alleles

作　　者：康军[1] 林晓凤[1] 

机构地区：[1]上海交通大学附属仁济医院南院检验科,上海201112

出　　处：《检验医学与临床》2016年第A02期203-206,共4页Laboratory Medicine and Clinic

摘　　要：目的探讨利用聚合酶链反应-序列特异性引物（PCR-SSP）技术检测TAP2等位基因多态性的方法及其可行性。方法用盐析法抽提随机健康人群DNA样本，以PCR—SSP技术为基础，根据TAP2＊0101，TAP2＊0102，TAP2＊0103，TAP2＊0201四种等位基因型的全序列特点，选取TAP2mRNA1308C—T，1693A—G，1951C—T等位基因上三个突变住点，使用primer5．0软件设计引物，通过PCR—SSP电泳获得等位基因不同的结果。结果根据PCR-SSP电泳结果获得的不同等位基因的格局图，清晰地得出每个个体的纯合子、杂合子情况，将TAP2所有类型完全分析清楚。结论该方法可确实有效地分型TPA2等位基因，成本低，操作方便，适合大规模批量地进行人群调查。此技术可用于人类群体遗传学调查，为研究TAP2基因多态性与疾病的关联性提供技术平台。Objective To explore the method and its feasibility of detecting the polymorphism of TAP2 alleles by PCR-SSP technology. Methods Random DNA samples of healthy people were salted out and extracted. According to the full sequence features of TAP2 ＊ 0101, TAP2＊ 0102, TAP2 ＊ 0103 and TAP2 ＊ 0201 alleles, three mutation sites of 1308C-T 1693A-G, TAP2mRNA,1951C-T were selected. Primers were designed by Primer5.0 software and PCR-SSP was performed to obtain all types of TAP2. Results According to the pattern of different alleles obtained by PCR-SSP electrophoresis, the homozygous and heterozygous status of each individual was clearly displayed,and all types of TAP2 were completely analyzed. Conclusion Different types of TPA2 alleles can be analyzed effectively and clearly by PCR-SSP with low cost and convenient operation. It is suitable for large-scale population investigation. This technique can be used in the study of human population genetics,which provides a technical platform for the study of the association between TAP2 gene polymorphism and diseases.

关 键 词：聚合酶链反应-序列特异性引物 TAP2 1308C—T 1693A-G 1951C-T 

分 类 号：R739.63[医药卫生—肿瘤]