G6P／6PG比值法用于筛查G6PD缺乏症的比对分析  

Comparative analysis of G6P / 6PG ratio method is used for screening G6PD deficiency

作　　者：陈奕微 迟绍琴[1] 孙宇飞[2] 

机构地区：[1]广东省深圳市龙岗区计划生育服务中心,518172 [2]广东省深圳华侨城医院,518053

出　　处：《检验医学与临床》2016年第A02期218-220,共3页Laboratory Medicine and Clinic

摘　　要：目的评价手工操作和全自动生化仪两种检测方法，对于改良葡萄糖6-磷酸脱氢酶定量比值法筛查G6PD缺乏症的一致性。方法收集患者样本，分别用手工操作法和全自动生化分析仪检测G6P／6PG比值，参照EP9-A2文件，进行方法学比对和偏倚评估。结果两种方法检测结果相关性分析尹为0．83，在医学决定水平0．95、1、1．05处，其偏倚为31．6％、29％、27．6％。两个方法间结果的偏倚不能接受。结论使用全自动生化分析仪上机检测G6P／6PG比值，可以提高G6PD缺乏症的检出率。采用手工法，必须做好方法间比对，建立自己实验室的参考区间。Objective Evaluation of manual operation and automatic biochemical analyzer two kinds of detection methods, for improving glucose 6 - phosphate dehydrogenase quantitative ratio method screening the consistency of G6PD deficiency. Methods Collect patient samples, respectively with manual operation and automatic biochemical analyzer test G6P/6PG ratio, consult EP9-A2 files,assess the methodological comparison and bias. Results Testing results of two methods of r^2 is 0.83, correlation analysis in medical decision level 0. 95,1,1. 05, the bias for 31.6 %, 29% and 31.6 %. The results between two methods of bias is unacceptable. Conclusion The full automatic biochemical analyzer were used to detect G6P/6PG ratio, can improve the detection rate of G6PD deficiency. Than using the manual method,the method must be done well, set up their own lab reference range.

关 键 词：定量比值法 G6PD缺乏症 比对实验 偏倍 

分 类 号：R596[医药卫生—内科学]