白藜芦醇通过抑制海马NF-κB减轻慢性坐骨神经挤压损伤大鼠神经病理性疼痛  

Ressveratrol facilitates neuropathic pain attenuation in a rat model through the inhibition of hippocampus NF-κB

作　　者：徐海龙[1] 齐敦益[2] 

机构地区：[1]江苏省麻醉与镇痛应用技术重点实验室徐州医学院江苏省麻醉学重点实验室,江苏徐州221004 [2]徐州医学院附属医院麻醉科,江苏徐州221002

出　　处：《临床和实验医学杂志》2017年第1期16-19,共4页Journal of Clinical and Experimental Medicine

摘　　要：目的评价白藜芦醇对大鼠神经病理性疼痛的治疗效应。方法雄性SD大鼠32只,随机分为4组(n=8):假手术+二甲基亚砜组(sham+DMSO)、假手术+白藜芦醇组(sham+Res)、慢性坐骨神经挤压损伤(CCI)+DMSO组、CCI+白藜芦醇组(CCI+Res)。假手术组仅暴露坐骨神经不做结扎,其他各组采用坐骨神经结扎术制备神经病理性痛模型,CCI术后5 d,分别经侧脑室注射DMSO 5μl和30μg Res(溶于DMSO 5μl),注射时间20 s,留针5 min。手术前,给药前,及侧脑室给药后1、7、14 d应用热辐射仪测定热缩足潜伏期(TWL),同时测定海马星型胶质细胞和NF-κB p65的表达变化。结果与sham+DMSO组相比,CCI+DMSO组各时间点TWL缩短,热痛阈(WTL)降低(P<0.05),CCI+Res与CCI+DMSO组相比TWL延长;同时海马星型胶质细胞活化受到抑制,NF-κB p65表达下调(P<0.05)。结论白芦藜醇可以减轻大鼠CCI神经病理性痛,其机制可能与抑制海马星型胶质细胞活化,抑制NF-κB p65表达有关。Objective To evaluate the effect of Ressveratrol on neuropathy pain in rats. Methods Thirty- two male wistar rats were randomly divided into 4 groups（ n = 8 each）,sham operation ＋ DMSO group（ sham ＋ DMSO）,sham ＋ Res group; chronic constrictive injury（ CCI） ＋ DMSO group（ CCI ＋ DMSO） and CCI ＋ Res group. Neuropahtic pain was induced by CCI,sham rats only underwent surgery of exposing the left sciatic nerve without ligation. On 5 d after CCI,30 μg Res（ in 5 μl of 5% DMSO） and 5% DMSO 5 μl were injected into the lateral cerebral ventricle over 20 s respectively,and the time of needle retaining was 5 min. paw withdrawal latency to themal stimulation（ TWL） was measured before operation,and at 1,7,12 and 14 d after intracerebroventricular injection. Meanwhile determinate the expression of NF- κB and the activation of astrocytes. Results Compared with sham group,TWL was shortened at any time in CCI ＋ DMSO groups（P〈0. 05）. Compared with CCI ＋ DMSO group,TWL was prolonged in group CCI ＋ Res（P〈0. 05）,meanwhile astrocytes in hippocampus were inhibited and the expression of NF- κB p65 was decreased（P〈0. 05）. Conclusion Resveratrol could reduce neuropathy pain,its mechanism may be related to inhibition of hippocampal astrocytes activation and the expression of NF- κB p65.

关 键 词：大鼠 慢性坐骨神经挤压损伤 神经病理性痛 白藜芦醇 星型胶质细胞 NF-ΚB p65 

分 类 号：R745.4[医药卫生—神经病学与精神病学]