健康儿童脂溶性维生素A、D、E现状分析  

Analysis of fat-soluble vitamins A, D, E levels in the serum of healthy children

作　　者：金春华[1,2] 王晓燕[1] 张丽丽[1] 张悦[1] 李娜[1] 王建红[1] 高海涛[1] 宋文红[1] 金娜[1] 高宝峻 蒋华英 王红 穆立娟 孙丽芳[5] 任霞 付迎新 郑春梅 倪君君 

机构地区：[1]首都儿科研究所儿童保健科,北京100020 [2]李桥儿童医院综合科,北京101314 [3]北京市平谷区妇幼保健院儿科,101200 [4]民航总医院儿科,北京100123 [5]北京市房山区妇幼保健院儿童保健科,102400 [6]北京市石景山区妇幼保健院儿童保健科,100040 [7]北京怀柔医院儿科,101400 [8]北京和合医学诊断技术股份有限公司中心实验室,101111

出　　处：《中华临床营养杂志》2016年第6期349-353,共5页Chinese Journal of Clinical Nutrition

基　　金：国家卫生计生委科技发展中心临床科研专项课题（W2015EAE001）

摘　　要：目的了解北京地区健康儿童血清维生素A、D、E水平。方法采集北京居住半年以上健康体检儿童的临床资料，利用反相高效液相色谱荧光检测法测定血清维生素A、维生素E，液相色谱串联质谱法测定血清25-羟维生素D[25-（OH）D]水平。结果健康体检儿童共1538例，维生素A平均（0．30±0．07）mg／L，亚临床缺乏占5．2％（80／1538），其中〈3岁占70．0％（56／80）；可疑亚临床缺乏占47．1％（724／1538），其中〈3岁占50．1％（363／724）。2岁及以下婴幼儿血清水平为（0．28±0．07）mg／L，处于可疑亚临床缺乏状态，母乳喂养儿维生素A水平最低（0．25±0．06）mg／L。268例儿童检测血清25-（OH）D，平均水平为（25．52±10．88）mg/L，缺乏占30．2％（81／268），其中≥3岁占92．6％（75／81）。血清维生素E平均（9．21±2．31）mg／L，正常占85．6％（1311／1532），缺乏仅为0．6％（9／1532），不足占13．8％（212／1532），且以〉3岁为主。维生素A、25-（OH）D、维生素E与年龄的Spearman相关系数分别为0．197、-0．420、-0．319。结论健康体检儿童维生素A以可疑亚临床缺乏为主，婴幼儿维生素D营养状况良好，学龄及青少年维生素D缺乏或不足问题明显，维生素E缺乏少见。Objective To investigate the serum levels of vitamin A, D, E in healthy children in Beijing. Methods We collected clinical data of healthy children who had been living in Beijing for more than six months. Their serum vitamin A and vitamin E levels were measured by reverse phase high-performance liquid chromatography （ HPLC ） with fluorescence detection method; serum 25-hydroxy vitamin D [ 25- （OH） D ] level was measured with liquid chromatography tandem mass spectrometry （HPLC-MS/MS）. Results A total of 1 538 healthy children were included. The average level of vitamin A was （0. 30 ± 0. 07） mg/L; subclinical deficiency rate was 5.2% （80/1 538） , of whom 70. 0% （56/80） were 〈 3 years; suspected subclinical deficiency rate was 47. 1% （724/1 538） , of whom 50. 1% （363/724） were 〈3 years. Infants under 2 years of age were in suspected subclinical deficiency state with an average serum concentration of （0. 28 ± 0. 07 ） mg/L. The lowest vitamin A level was observed in breastfed children [ （0. 25 ±0. 06） mg/L]. The average level of serum 25- （OH） D in the 268 children detected was （ 25.52 ± 10. 88 ） ng/L, vitamin D deficiency was observed in 30. 2% （81/268） of the children, of whom 92. 6% （75/81） were ≥3 years. The mean serum vitamin E level was （9. 21 ±2. 31 ） mg/L, 85.6% （ 1 311/1 532） were normal, 0. 6% （9/1 532） were deficient, and 13.8% （212/1 532） were insufficient, who were mainly 〉 3 years. Spearman＇ s correlation coefficients between the serum vitamin A, 25-（OH）D, vitamin E and age were 0. 197, -0. 420, and -0. 319, respectively. Conclusions Serum vitamin A level in healthy children may be mainly in suspected subclinical deficiency state. Infants generally in good vitamin D nutritional status, while vitamin D deficiency or insufficiency is more prevalent in school-age children and teenagers. Vitamin E deficiency is rare.

关 键 词：儿童 维生素A 维生素E 25-羟维生素D 缺乏症 

分 类 号：R446.11[医药卫生—诊断学]