八宝丹处理肺腺癌细胞A549和SPCA-1对顺铂化疗敏感性的影响  

Effect on sensitivity to cisplatin after Babaodan treatment of human lung adenocarcinoma cells A549 and SPCA-1

作　　者：成瑜[1] 李洪伦[2] 

机构地区：[1]青岛大学附属烟台毓璜顶医院肿瘤内科,烟台264000 [2]青岛大学附属烟台毓璜顶医院影像中心

出　　处：《滨州医学院学报》2016年第6期414-417,共4页Journal of Binzhou Medical University

基　　金：烟台市发展计划项目(2012092)

摘　　要：目的探讨八宝丹处理人肺腺癌A549和SPCA-1细胞系对顺铂(DDP)抗肿瘤化疗敏感性影响。方法收集人肺腺癌A549和SPCA-1细胞系进行不同处理,随机分为对照组(空白)、DDP组(4uM)、(八宝丹+DDP)组(0.75mg/kg+4uM),处理3周,采用MTT法检测细胞增殖能力;末端脱氧核苷酸转移酶介导的d UTP缺口末端标记测定(TUNEL)法检测细胞凋亡率,采用transwell检测细胞迁移能力。结果与对照组比较,DDP组和(八宝丹+DDP)组的A549和SPCA-1细胞增殖能力明显降低(P<0.01);与DDP组比较,(八宝丹+DDP)组的A549和SPCA-1细胞增殖能力无明显差异(P>0.05)。与对照组比较,DDP组和(八宝丹+DDP)组的A549和SPCA-1细胞凋亡明显增加(P<0.01);与DDP组比较,(八宝丹+DDP)组的A549和SPCA-1细胞凋亡明显增加(P<0.05)。与对照组比较,DDP组和(八宝丹+DDP)组的A549和SPCA-1细胞迁移数目明显降低(P<0.01);与DDP组比较,(八宝丹+DDP)组的A549和SPCA-1细胞迁移数目明显降低(P<0.001)。结论(八宝丹+DDP)可以增加肿瘤细胞的凋亡,降低肿瘤细胞的增殖,减少A549和SPCA-1细胞迁移数目,增加肿瘤细胞的DDP抗肿瘤敏感性。Ojeive o udy he odn emen of humn lung denoinom ell line 549 nd line P-1o iplin（DDP）hemohepy eniiviy.Mehod olleed humn lung denoinom 549 nd P-1ell line fo diffeen emen,wee ndomly divided o onol goup（o no）,DDP goup（4 UM）,odn DDP goup（0.75 mg/kg＋4UM）,emen w 3week,ued M y o dee he iliy of ell polifeion,ued eminl deoxynuleoidyl nfe enzyme medied D UP nik end leling deeminion（UNEL）mehod o dee popoi e,ued nwell y o dee he iliy of ell migion.eul omped wih he onol goup,he 549 nd P-1ell polifeion of DDP goup nd odn＋DDP goup deeed ignifinly（P〈0.01）;omped wih he DDP goup,he 549 nd P-1ell polifeion of odn＋DDP goup hd no ignifin diffeene（P〉0.05）.nd omped o he onol goup,he DDP goup nd odn＋DDP goup of 549 nd P--1ell popoi ineed ignifinly（P〈0.01）;omped wih he DDP goup,odn＋DDP goup on 549 nd P--1ell popoi ineed ignifinly（P〈0.05）.nd omped o he onol goup,549 nd P--1ell migion nume of DDP goup nd odn＋DDP goup deeed ignifinly（P〈0.01）;omped wih he DDP goup,odn＋DDP goup on 549 nd P--1ell migion nume deeed ignifinly（P〈0.001）.onluion odn DDP n inee he popoi of ne ell,edue he polifeion of ne ell,edue ell nd he nume of miged of he 549 nd P--1,nd enhne he hemoheopy eniiviy of DDP.

关 键 词：肺腺癌 八宝丹 顺铂 化疗敏感性 

分 类 号：R734.2[医药卫生—肿瘤]