miR-1通过调控神经嵴细胞增殖和凋亡影响颌骨发育  

microRNA-1 affects the development of the jawregulates by regulating the apoptosis and proliferation of the neural crest cells

作　　者：王冬玥 翁亚娟[2] 倪洁丽[2] 郭舒瑜 马俊青[1,2] WANG Dongyue WENG Yajuan NI Jleli GUO Shuyu MA Junqing(Jiangsu Key Laboratory of Oral Diseases, Nanfing Medical University, Nanjing 210029, China Department of Orthodontics, Affiliated Hospital of Stomatology, Nanjing Medical Uni- versity, Nanjing 210029, China)

机构地区：[1]南京医科大学口腔疾病研究江苏省重点实验室,江苏南京210029 [2]南京医科大学附属口腔医院正畸科,江苏南京210029

出　　处：《口腔生物医学》2016年第4期169-172,共4页Oral Biomedicine

基　　金：国家自然科学基金资助项目(81371179);江苏高校优势学科建设工程资助项目(2014-37)

摘　　要：目的:研究微小RNA-1(miR-1)在斑马鱼胚胎发育过程中对神经嵴的影响。方法:通过显微注射miR-1反义核苷酸(MO)抑制miR-1的功能,在胚胎发育96 h时,观察下颌骨的发育,软骨染色观察颅面部软骨的发育状况。TUNEL和磷酸化组蛋白H3(p H3)免疫荧光观察神经嵴细胞的凋亡和增殖。结果:在miR-1被敲低后,下颌发育不足,软骨染色显示下颌骨和舌骨形态结构异常。神经嵴细胞的凋亡异常增多,增殖能力降低。结论:miR-1参与调节了颅面部神经嵴细胞的发育,miR-1对于神经嵴细胞的活性具有调控作用,导致颅面部发育过程中神经嵴来源的下颌骨发育缺陷。Objective：To explore the effect of miR-1 on the development of neural crest in zebrafish.Methods：We observed the development of mandible and cranial cartilages at 96 hours post fertilization（hpf） after knockdown the miR-1.Then we carried out the TUNEL and immunofluorescence of phosphorylation of histone H3（pH3） to study the apoptosis and proliferation of the neural crest cells.Results：In this study,it is demonstrated that knockdown of miR-1 resulted in the impaired craniofacial chondrogenesis.We observed reduced lower jaw,abnormal mandibular and ceratohyal cartilage.We also found knockdown of miR-1 promoted the cell death and suppressed the proliferation during the development of neural crest cells.Conclusions：These results indicate an important role for miR-1 in neural crest cells development and suggest involvement of miR-1 in neural crest cell viability.

关 键 词：微小RNA-1 神经嵴 凋亡 增殖 斑马鱼 

分 类 号：R780.2[医药卫生—口腔医学]