异种脱细胞真皮基质对新生血管作用的研究  

In vitro study of the effect of xeno-acellular dermal matrix on vasculogenesis

作　　者：达静姝 陈武[1,3] DA Jingshu CHEN Wu(Jiangsu Key Laboratory of Oral Diseases, Nanfing Medical University, Nanjing 210029, China The Third Clinic, Affiliated Hospital of Stomatology , Nanfing Medical University, Nanjing 210014, China Department of Periodon- tics, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, China)

机构地区：[1]南京医科大学口腔疾病研究江苏省重点实验室,江苏南京210029 [2]南京医科大学附属口腔医院第三门诊部,江苏南京210014 [3]南京医科大学附属口腔医院牙周病科,江苏南京210029

出　　处：《口腔生物医学》2016年第4期187-190,共4页Oral Biomedicine

基　　金：江苏省卫生科技计划项目(H201231);江苏高校优势学科建设工程资助项目(2014-37)

摘　　要：目的：探讨不同异种脱细胞真皮基质对内皮细胞血管生成的促进作用。方法：利用人脐静脉内皮细胞（HUVEC）,与猪脱细胞真皮基质（P-ADM）和牛脱细胞真皮基质（B-ADM）共培养,以Bio-Gide可吸收生物膜和空白组作对照,ELISA法分别检测细胞接种4、6、12、24、48、96 h共培物上清中血管内皮生长因子（VEGF）的含量;GFP慢病毒转染于HUVEC后,在倒置相差显微镜下计数共培物上细胞管型的数目。结果：在细胞接种24 h时,ADM组与Bio-Gide组和空白组比较VEGF的浓度更高（P〈0.05）;48 h时P-ADM组VEGF的浓度高于与Bio-Gide组和空白组（P〈0.05）。转染GFP慢病毒的HUVEC与生物膜共培养6～48 h时,B-ADM组细胞管型多于P-ADM组和Bio-Gide组;24 h内,P-ADM组多于Bio-Gide组;空白组未见明显管型。结论：P-ADM与B-ADM比Bio-Gide具有更好的成血管作用,且B-ADM效果更明显,提示异种ADM有较好的促新生血管的作用,可作为替代物应用于膜龈手术。Objective：To research the effect of xeno-acellular dermal matrix（xeno-ADM） on vasculogenesis of human umbilical vein endothelial cell（HUVEC）.Methods：Porcine acellular dermal matrix（P-ADM） and bovine acellular dermal matrix（B-ADM）were co-cultured with HUVEC,using blank and Medical collagen membrane（Bio-Gide） as control.The concentration of vascular endothelial growth factor（VEGF） collected from different groups at 4,6,12,24,48,96 h was determined by ELISA test.Meanwhile,the number of cellular casts were counted by observing HUVEC infected by GFP expression lentivirus.Results：The VEGF concentration of xeno-ADM groups were higher than that of the control groups（P〈0.05） after 24 h,and P-ADM was more than that of the control groups after 48 h（P〈0.05）.The number of cellular casts of P-ADM group was about as twice as that of the control group after 24 h.The B-ADM group improved less than the P-ADM group.No cellular cast was found in the control group.Conclusions：Xeno-ADM has better effect on vasculogenesis than traditional medical collagen membrane,which suggesting that it has the potential to be the next generation of surgical material applying to mucogingival therapy.

关 键 词：异种脱细胞真皮基质 人脐静脉内皮细胞 血管内皮生长因子 细胞管型 

分 类 号：R780.2[医药卫生—口腔医学]