槟榔碱和Ca^(2+)对口腔黏膜异常增生上皮体外模型通透性的影响  

Effects of arecoline and calcium ion on the permeability of dysplastic oral epithelia model in vitro

作　　者：侯冬兰[1] 陈蓉[1] 高义军[1] 尹晓敏[2] 

机构地区：[1]中南大学湘雅二医院口腔医疗中心,湖南长沙410011 [2]中南大学湘雅医院口腔医学中心,湖南长沙410008

出　　处：《口腔疾病防治》2017年第1期21-25,共5页Journal of Prevention and Treatment for Stomatological Diseases

基　　金：湖南省自然科学基金资助项目(14JJ2030)

摘　　要：目的研究槟榔碱对口腔黏膜异常增生上皮通透性的影响及Ca^(2+)对其的调节作用。方法建立口腔黏膜异常增生上皮体外细胞模型;设置对照组、槟榔碱处理组及Ca^(2+)+槟榔碱处理组,以荧光黄表观通透系数(Papp)评价槟榔碱及Ca^(2+)对细胞模型通透性的影响。结果在槟榔碱处理组,各亚组荧光黄Papp均大于相应对照组(P<0.05),且荧光黄Papp随槟榔碱浓度及干预时间增加而增大(P<0.05)。在Ca^(2+)+槟榔碱处理组,10μg/m L槟榔碱浓度下各亚组荧光黄Papp分别比相同浓度和时间点的槟榔碱处理组低(P>0.05),4 h 10μg/m L小组的荧光黄Papp与相应对照组的差异无统计学意义(P>0.05),其他各小组荧光黄Papp均大于对照组(P<0.05),且荧光黄Papp随槟榔碱浓度及干预时间增加而增大(P<0.05)。结论槟榔碱干预使细胞模型通透性增大,可能是上皮异常增生组织癌变的重要原因之一,钙离子预处理使槟榔碱增加上皮通透性的作用减弱。Objective To study the effects of arecolineand Calcium ion（Ca^2＋） on the permeability of dysplastic oralepithelia model in vitro. Methods To establish the dysplastic oral epithelia model in vitro by culturing oral keratinocyte（DOK） and harvesting a DOK cell monolayer. The models were divided into control group, arecoline group and ＂Ca^2＋＋ are-coline＂ group. The values of Lucifer Yellow Pappwere used to estimate the permeability changes of models after treated-with arecoline and Ca^2＋. Results In arecoline group, the lucifer yellow Pappvalues of each subgroup were higher than thatof control group（P〈0.05）, and the values increased as arecoline concentration elevated and time lasted（P〈0.05）.When the ＂Ca^2＋＋ arecoline＂ group were pretreated with Ca^2＋, the values of subgroup ＂10 μg/m L＂ was lower than that ofthe corresponding arecoline group（P〈0.05）. However, the value of subgroup ＂4 h 10 μg/m L＂ of ＂Ca^2＋＋ arecoline＂ grouphad no statistical difference with that of control group（P〉0.05）, while the other subgroups were increased（P〈0.05）; Be-sides, these values in ＂Ca^2＋＋ arecoline＂ group were increased as the arecoline concentration elevated and time lasted too（P〈0.05）. Conclusion Intervention of arecoline contributes to the increase of permeability of DOK cell monolayer mod-el, which maybe an important reason for the cancerization of dysplastic oral epithelia, however Ca^2＋might weaken these ef-fects of arecoline in the process.

关 键 词：槟榔碱 口腔黏膜异常增生上皮 体外模型 CA2+ 通透性 

分 类 号：R781.5[医药卫生—口腔医学]