一株降解微囊藻毒素的缺陷短波单胞菌的分离鉴定及降解特性研究  

Isolation,identification and characterization of a novel microcystin-degrading bacterium

作　　者：谷青[1] 赵琪[1] 史全良[1] 

机构地区：[1]苏州大学基础医学与生物科学学院,苏州215123

出　　处：《环境科学学报》2017年第1期201-206,共6页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金(No.31171467)~~

摘　　要：从太湖水华腐烂蓝藻中筛选出一株能够降解MC-LR的细菌,将其编号为Q3.经形态、生理生化及16S rRNA基因序列分析鉴定为缺陷短波单胞菌(Brevundimonas diminuta).研究发现,在实验条件下该菌能以MC-LR为唯一碳源和氮源生长;7 d内可将初始浓度为0.96 mg·L-1的MC-LR降解为0.37 mg·L-1,降解效率达到61.5%.同时,本研究首次发现缺陷短波单胞菌能够降解藻毒素,并且在此菌种中扩增出了降解过程的关键基因mlr A,推测该菌可能与已报道的Sphingomonas sp.ACM-3962等菌具有相同的降解机制.A novel microcystin-degrading bacterium Q3 is isolated from rotten cyanobacteria of Taihu Lake. According to the analysis of morphology,physiological characteristics,as well as 16 S rRNA gene sequencing,it could be identified as Brevundimonas diminuta. This bacterium could use microcystin-LR as the sole carbon and nitrogen sources for growth. More importantly,this bacterium was capable of degrading MCs from the initial concentration of 0.96 mg·L-1to 0.37 mg·L-1during 7 days of incubation,with The degradation efficiency up to 61.5%. It is the first time to report that Brevundimonas diminuta could effectively degrade MCs. In addition,the mlr A gene,as a key enzyme for MCs degradation,was found and amplified in this strain. These results show that the MCs degradation pathway by Q3 is similar to that of Sphingomonas sp.ACM-3962.

关 键 词：微囊藻毒素 生物降解 mlrA基因 缺陷短波单胞菌 

分 类 号：X172[环境科学与工程—环境科学]