支架蛋白RACK1小干扰RNA抑制卵巢癌细胞CAOV3的增殖、迁移和侵袭  

Small interfering RNA of RACK1 inhibiting cell proliferation,migration and invasion in ovarian cancer CAOV3 cells

作　　者：孙静 任丽莉 张晓玉 刘超 

机构地区：[1]锦州医科大学基础医学院发育生物学教研室,辽宁锦州121001 [2]锦州医科大学基础医学院神经生物学教研室

出　　处：《中国老年学杂志》2017年第2期263-266,共4页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(31371173);辽宁省大学生创新创业训练计划项目(201310160021)

摘　　要：目的观察支架蛋白RACK1小干扰RNA(siRNA)对卵巢癌CAOV3细胞增殖、迁移和侵袭的影响和基质金属蛋白酶(MMP)-2和MMP-9的表达变化。方法体外培养CAOV3细胞,实验分scramble siRNA组和RACK1 siRNA组;Lipofectamine 2000转染CAOV3细胞,Western印迹检测RACK1的干涉效能;MTT法测定CAOV3细胞的增殖率;划痕实验和transwell迁移和侵袭实验研究RACK1对细胞体外增殖、迁移和侵袭运动能力的影响;Western印迹检测CAOV3细胞中MMP-2和MMP-9蛋白表达。结果与scramble siRNA组比较,RACK1 siRNA组RACK1的蛋白表达水平降低,明显抑制CAOV3细胞的增殖、迁移和侵袭,降低MMP-2和MMP-9蛋白表达。结论下调RACK1表达可抑制卵巢癌细胞CAOV3的增殖、迁移和侵袭,其机制可能与改变MMP-2和MMP-9蛋白表达相关。Objective To observe the effects of double-stranded small interfering RNA （siRNA） of scaffolding protein RACK1 on the cell proliferation, migration, invasion of ovarian cancer CAOV3 cells and the protein expression levels of matrix metalloproteinases MMP-2 and MMP-9 in CAOV3 cells. Methods CAOV3 cells were cultured in vitro and transfected with RACK1 siRNA and scramble siRNA ran- domly. The transfected efficiency of RACK1 siRNA was determined by Western blot, the proliferation rate of CAOV3 cells was assayed by MTT,the cell migration and invasion of CAOV3 cells were examined by wound healing, transwell migration and invasion experiment. The MMP-2 and MMP-9 protein expression levels were detected by Western blot. Results Compared with scramble siRNA group, RACK1 siRNA inhibited the proliferation, migration and invasion of CAOV3 cells and decreased the protein expression of MMP-2 and MMP-9. Conclusions RACK1 silencing decreases the proliferation, migration and invasion of ovarian cancer CAOV3 cells via down-regulation of the protein ex- pression of MMP-2 and MMP-9 in CAOV3 cells.

关 键 词：RACK1 siRNA 卵巢癌 CAOV3 

分 类 号：R581.3[医药卫生—内分泌]