miR-126、miR-355及Exportin-5在肺癌中表达研究  

Expression of miR-126, miR-355 and Exportin-5 in lung cancer

作　　者：张若冰[1] 杨凯云[2] 谭慧[2] 平妮娜[1] 姚树祥[1] 吴锡南[1] 何越峰[1] 

机构地区：[1]昆明医科大学公共卫生学院,650500 [2]昆明医科大学第三附属医院胸心外科,650118

出　　处：《实用医学杂志》2017年第1期44-47,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金(编号:81460491);云南省卫生厅内设机构项目(编号:2016NS108)

摘　　要：目的:探讨miR-126、miR-355及Exportin-5在肺癌中表达及相互之间关系。方法:收集47例肺癌患者的癌组织及癌旁组织,利用TRIzol提取总RNA,使用定时荧光定量PCR检测基因的相对表达量。结果:肺癌组织与癌旁组织中的miR-126,Exportin-5差异具有统计学意义(P<0.05),癌旁组织中miR-126与pri-miR-126(r=-0.31)呈负相关(P<0.05),癌组织中基因表达量与临床指标之间的关系中发现miR-126与MRP(r=0.43)呈正相关(P<0.05),Exportin-5与TOPO(r=-0.36)、pri-miR-126与饮酒(r=-0.34)、MRP(r=-0.43)呈负相关(P<0.05)。结论:miR-126、Exportin-5在肺癌组织中表达下调,基因在肺癌发生发展过程中可能通过抑制基因的表达促进肺癌的发生与发展。Objective To investigate the expression of miR-126, miR-355 and exportin-5 in lung cancer. Methods The cancer tissue and the tissue adjacent to carcinoma of 47 cases of patients with lung cancer was used to detect the expression of miR-126, miR-355 and Exportin-5 by the real-time fluorescence quantitative PCR. Results Significant difference of the expression of miR- 126 （t = 2.02, P = 0.03） and exportin-5 （t = 4.62, P 〈 0.01） was observed in lung cancer tissue and tissue adjacent to carcinoma. Mature miR-126 and pri-miR-126 （R = 0.309, P = 0.044） had a negative correlation in the tissue adjacent to carcinoma. In the cancer tissue,miR-126 and MRP （R = 0.432, P = 0.019）, miR-335 and k167 （R = 0.410, P = 0.033） were positively correlated, however, exportin-5 and TOPO （R = 0.357, P = 0.045）, the pri-miR-126 and drinking （R = 0.340, P = 0.024）, the pri-miR-126 and MRP （R = 0.427, P = 0.027） had a negative correlation relationship. Conclusion Expression of miR-126 and exportin-5 was decreased in lung cancer tissue, which may contribute to the occurrence and development of lung cancer.

关 键 词：肺肿瘤 MIR-126 miR-355 Exportin-5 

分 类 号：R734.2[医药卫生—肿瘤]