肝素结合表皮生长因子对肝移植模型大鼠肝脏炎症因子及细胞再生的影响  

Effect of heparin binding epidermal growth factor-like growth factor on hepatic inflammatory cytokines and cell regeneration in rat liver transplantation model

作　　者：周立新[1] 苏继钦 王茂林[1] 严辉弟[1] 林培艺[1] 

机构地区：[1]厦门市第二医院肝胆外科,福建厦门361021

出　　处：《胃肠病学和肝病学杂志》2016年第12期1463-1466,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：厦门市科技计划指导性项目(3502Z20139012)

摘　　要：目的研究肝素结合表皮生长因子(heparin-binding epidermal growth factor-like growth factor,HB-EGF)对肝移植模型大鼠肝脏炎症因子及细胞再生的影响。方法选择60只SD雄性大鼠并建立肝移植动物模型,随机分为A、B、C三组,B组皮下注入0.1mg/kg肝细胞生长因子,C组皮下注射0.1 mg/kg HB-EGF,A组给予相等剂量生理盐水进行干预。干预后第1、3、5、7天时,比较三组肝脏湿重、肝功能、炎性因子含量。结果干预后第1、3、5、7天时,B组、C组大鼠移植肝脏的湿重均明显高于A组(P<0.05),血清丙氨酸氨基转氨酶(ALT)、总胆红素(TBIL)含量均明显低于A组(P<0.05);干预后第3、5、7天时,B组、C组血清白蛋白(ALB)含量明显高于A组(P<0.05);干预后第1、3、5、7天时,B组、C组大鼠肝脏组织中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)含量均明显低于A组(P<0.05);C组大鼠肝脏组织中TNF-α、IL-6含量明显低于B组(P<0.05)。结论 HB-EGF能促进肝移植模型大鼠肝细胞再生,其作用机制可能与保护肝功能、减轻炎症反应有关。Objective To study the effect of heparin binding epidermal growth factor-like growth factor （HB-EGF） on hepatic inflammatory cytokines and cell regeneration in rat liver transplantation model. Methods Sixty male SD rats were established animal model of liver transplantation, and randomly divided into group A, group B, group C. Group B subcutaneous injection O. 1 mg/kg hepatocyte growth factor, group C subcutaneous injection O. 1 mg/kg HB-EGF, group A was given equal dose of normal saline to intervene. On the 1 st, 3rd, 5th, 7th day after intervention, liver wet weight, liver function, liver inflammatory cytokines levels were compared among three groups. Results On the 1st, 3rd, 5th, 7th day after intervention, rats liver wet weight in group B, group C were significantly higher than those in group A （P 〈 O. 05）. Serum ALT, TBIL were significantly lower than those in group A （P 〈 0.05） ; on the 3rd, 5th, 7th day after intervention, serum ALB in group B, group C was significantly higher than that in group A （P 〈 0.05） ; on the 1 st, 3rd, 5th, 7th day after intervention, liver tissue TNF-α, IL-6 in group B, group C were signficantly lower than those in group A （P 〈 0.05 ） ; liver tissue TNF-α, IL-6 in group C were signficantly lower than those in group B （P 〈 0.05 ）. Conclusion HB-EGF can promote liver transplantation model of rat liver cell regeneration, the mechanism may be re- lated to the protection of liver function, and the reduction inflammation reactions.

关 键 词：肝移植 肝细胞生长因子 肝素结合表皮生长因子 细胞再生 炎症因子 

分 类 号：R575[医药卫生—消化系统]