miR-1288调控骨髓间质干细胞促进胃癌细胞迁移  

Bone marrow-derived mesenchymal stem cell promotes migration of human gastric cancer cell by regulation of miR-1288

作　　者：田伊卿[1] 曹晓莉[2] 李权[1] 王姣姣[1] 许文荣[1] 王梅[1] 

机构地区：[1]江苏大学医学院,江苏镇江212013 [2]南通市肿瘤医院检验科,江苏南通226361

出　　处：《临床检验杂志》2016年第10期758-761,共4页Chinese Journal of Clinical Laboratory Science

基　　金：国家自然科学基金(81302119);江苏省自然科学基金(BK20130540);江苏大学高级专业人才科研启动基金(13JDG088);江苏大学青年骨干教师培养工程

摘　　要：目的探讨miR-1288在胃癌细胞培养上清诱导后骨髓间质干细胞(bone marrow-derived mesenchymal stem cell,BMMSC)中的表达情况及其调控BMMSC对胃癌细胞迁移能力的影响。方法采用SGC-7901、MGC-803和HGC-27 3种胃癌细胞培养上清分别处理BMMSC,实时荧光定量PCR检测处理前后BMMSC中miR-1288的表达水平。采用寡核苷酸片段NC-mimics和miR-1288-mimics转染BMMSC,Transwell试验检测转染后BMMSC对HGC-27细胞迁移能力的影响。收集胃炎和胃癌组织标本,实时荧光定量PCR检测组织中miR-1288的表达水平。结果经SGC-7901、MGC-803和HGC-27胃癌细胞上清处理后BMMSC中miR-1288的表达水平分别是对照组的(5.91±0.25)倍(t=15.55,P<0.01)、(9.69±0.62)倍(t=13.34,P<0.01)和(24.29±2.69)倍(t=8.631,P<0.01)。miR-1288-mimics转染组迁移细胞数量为(623.3±26.03)个,明显高于NCmimics对照组[(326±32.08)个],差异有统计学意义(t=7.197,P<0.01)。胃癌组织中miR-1288的表达水平显著高于胃炎组织(P<0.05)。结论胃癌上清诱导后BMMSC和胃癌组织中异常高表达miR-1288,其可参与调控胃癌细胞的迁移。Objective To explore the expression levels of miR-1288 in bone marrow-derived mesenchymal stem cell（BMMSC） treated with gastric cancer cell conditioned medium（CM） and the role of miR-1288 in BMMSC-regulating migration of human gastric cancer cell. Methods BMMSC were treated with CM from three gastric cancer cell line, i.e. , SGC-7901, MGC-803 and HGC-27, respec- tively, and the expression levels of miR-1288 in BMMSC were detected by quantitative real-time PCR（qRT-PCR）. BMMSC were transfected with oligonucleotide segments of negative control（NC）-mimics and miR-1288-mimics respectively. Transwell migration as- say was used to explore the effects of BMMSC on the migration ability of gastric cancer cell HGC-27 after transfection. The tissues of gastritis and gastric cancer were collected and the expression levels of miR-1288 were measured by qRT-PCR. Results The fold change of miR-1288 expression in BMMSC treated with SGC-7901, MGC-803 and HGC-27 were （5.91 ± 0.25） （ t = 15.55, P 〈 0.01 ）, （ 9.69 ± 0.62） （ t = 13.34, P 〈 0.01 ） and （ 24.29 ± 2.69 ） （ t = 8.631, P 〈 0.01 ） respectively, which was uniformly higher than those of the control group. The number of migrating cells in miR-1288-mimics group was （623.3 ± 26.03 ） which was significantly more than those （326 ± 32.08） in NC-mimics group （ t = 7. 197, P 〈 0. 01 ）. miR-1288 expression level in gastric cancer tissues was significantly higher than that of gastritis tissue（P 〈 0.05）. Conclusion miR-1288 may be highly up-regulated in gastric cancer cell conditioned medium-inducing BMMSC and gastric cancer tissues, and participate in the regulation of gastric cancer cell migration.

关 键 词：胃癌微环境 骨髓间质干细胞 miR-1288 胃癌 迁移 

分 类 号：R735.2[医药卫生—肿瘤]