经口灌胃卵清蛋白对哺乳期BN大鼠致敏性的影响  

Effect of oral ovalbumin exposure on allergy in BN rats during maternal milk feeding

作　　者：龙再浩 孙运 王建峰 章军[2] 

机构地区：[1]宁波检验检疫科学技术研究院,浙江宁波315012 [2]浙江大学医学院毒理教研室

出　　处：《毒理学杂志》2016年第6期431-434,共4页Journal of Toxicology

基　　金：宁波市自然科学基金(2014A610287)

摘　　要：目的使用卵清蛋白经口灌胃致敏不同周龄的BN大鼠,初步探讨哺乳期暴露致敏蛋白对BN大鼠致敏性影响,并了解血清中细胞因子变化。方法选2周龄、4周龄和8周的BN大鼠,经口灌胃1 mg/kg·bw·d剂量卵清蛋白,连续35 d。第28天和第35天时内眦静脉采血,采用酶联免疫吸附法测定血清中OVA特异性Ig E(s Ig E)抗体、细胞因子IL-4、IL-10和IFN-γ浓度。结果第28天时,8周龄组BN大鼠血清中OVA s Ig E浓度显著高于对照组(P<0.05),2周龄和4周龄组与对照组之间差异无统计学意义(P>0.05)。第35天时,4周龄组和8周龄组大鼠血清中OVA s Ig E浓度都显著高于对照组(P<0.05),2周龄组与对照组比较差异无统计学意义。同时第35天细胞因子检测结果,2周龄组BN大鼠血清中IL-10和IFN-γ浓度降低(P<0.01),IL-4浓度变化不大。4周龄组和8周龄组BN大鼠血清中IL-4和IL-10浓度增加(P<0.05),血清中IFN-γ浓度变化不大。结论哺乳期会抑制卵清蛋白致敏BN大鼠,这可能受到细胞因子影响。Objective To investigate the effect of immune response after oral ovalbumin（OVA） exposure during maternal milk feeding on Brown Norway（BN） rat pups as well as cytokines. Methods Different age rats（2,4 and 8 weeks） were used to treat with1 mg/d of OVA by gavage for 35 days. The levels of OVA specific serum Ig E,IL-4,IL-10 and IFN-γ were analyzed on the 28 thday and 35 th day by enzyme linked immunosorbent assay（ELISA）. Results On day 28,8 week group rat produced significantly higher concentration of OVA sIgE compared to the control group（P〈0. 05）. On day 35,the concentrations of OVA sIgE in 4 week group and 8 week group were significantly higher than control group（P〈0. 05）. In addition,The treatment of OVA significantly reduced the concentrations of IL-10 and IFN-γ in 2 week group（P〈 0. 01）,and increased the concentrations of IL-4 and IL-10 in 4 week and 8 week group on day 35. Conclusion The oral OVA exposure could restrain the development of food sensitization during maternal milk feeding in BN rats,which may be related to cytokines.

关 键 词：BN大鼠 食物过敏 动物模型 

分 类 号：R155.3[医药卫生—营养与食品卫生学]