miR-181a在肾透明细胞癌中的表达及对肾透明细胞癌细胞786-O功能的影响  

Expression of miR-181a in human clear cell renal carcinoma and its effect on biological behavior of clear cell renal carcinoma 786-O cells

作　　者：雷振伟[1] 张瑜[1] 高宇[1] 范阳[1] 李新涛[1] 陈路遥[1] 张帆[1] 陈建文[1] 唐露[1] 巫胜攀 王瀚锋[1] 张旭[1] LEI Zhenwei ZHANG Yu GAO Yu FAN Yang LI Xintao CHEN Luyao ZHANG Fan CHEN Jianwen TANG Lu WU Shengpan WANG Hanfeng ZHANG Xu(Department of Urology, Chinese PLA General Hospital, Beijing 100853, China)

机构地区：[1]解放军总医院泌尿外科,北京100853

出　　处：《解放军医学院学报》2016年第12期1273-1277,1292,共6页Academic Journal of Chinese PLA Medical School

基　　金：国家高技术研究发展计划(863)资助课题(2014AA020607)~~

摘　　要：目的探讨miR-181a在肾透明细胞癌中的表达及miR-181a对肾透明细胞癌细胞系786-O功能的影响。方法通过q RT-PCR的方法检测miR-181a在42对肾透明细胞癌肿瘤及瘤旁组织和细胞系786-O、769-P、A498、Caki-1中的表达水平;以miR-181a mimics、miR-181a inhibitor转染786-O细胞系,通过MTS实验观察其增殖能力的改变,并通过流式细胞仪检测细胞周期及细胞凋亡的变化。结果 miR-181a在肾透明细胞癌中的表达明显高于瘤旁组织,同时其在细胞系786-O、769-P、A498、Caki-1中的表达明显高于其在人肾小管上皮细胞HKC中的表达。在786-O细胞系中,转染miR-181a mimics可以明显促进其增殖,诱导G1/S期转换并抑制凋亡;而miR-181a inhibitor则抑制增殖,诱导G1期阻滞并促进凋亡。结论 miR-181a在肾透明细胞癌中高表达并参与肿瘤的发生和发展,提示miR-181a可能成为肾癌治疗的潜在靶点。Objective To investigate the expression of miR-181 a in patients with clear cell renal carcinoma（cc RCC） and its effect on biological behaviors of human cc RCC cell line 786-O. Methods The expression of miR-181 a in 42 tumor tissues and paired adjacent normal tissues of cc RCC, and cc RCC cell lines 786-O, 769-P, A498, caki-1 were detected by q RT-PCR. Chemically synthesized miR-181 a mimics or inhibitor were transfected into cc RCC cell line 786-O, then its proliferation was observed by MTS assay and its cell cycle and apoptosis were detected by flow cytometric analysis. Results Compared with adjacent noncancerous tissues, miR-181 a expression increased significantly in cc RCC tissues, and it was also significantly higher in cell lines 786-O, 769-P, A498, caki-1 than in human kidney tubule epithelial cell（HKC）. miR-181 a mimics significantly promoted cell proliferation, G1/S transition in cell cycle and inhibited cell apoptosis in cc RCC cell line 786-O, however, miR-181 a inhibitor significantly inhibited cell proliferation, arrested cells in G1 phase while promoted cell apoptosis. Conclusion The miR-181 a expresses highly in cc RCC and also participates in the occurrence and development of tumor, which may be a valuable prognostic predictor and a potential therapeutic target of cc RCC.

关 键 词：miR-181a 肾透明细胞癌 786-O细胞 

分 类 号：R737.11[医药卫生—肿瘤]