青藏高原青稞耐寒种质资源基于SSR标记的遗传多样性及群体结构分析  

Genetic Diversity and Population Structure Analysis of Hulless Barley with Cold Tolerance from the Qinghai-Tibetan Plateau Revealed by SSR Markers

作　　者：巴桑玉珍[1,2] 刘新春[1] 付国勇[1] 李东梅[1] 王丹丹[1] 强小林[2] 冯宗云[1] 

机构地区：[1]四川农业大学农学院作物遗传育种学系大麦青稞研究中心,四川成都611130 [2]西藏自治区农牧科学院农业研究所,西藏拉萨850032

出　　处：《麦类作物学报》2017年第1期40-47,共8页Journal of Triticeae Crops

基　　金：国家现代农业产业技术体系(大麦青稞)建设专项(CARS-05)

摘　　要：为了解青藏高原青稞耐寒种质资源的遗传基础,利用SSR标记分析了来自青藏高原地区的71份青稞耐寒育种资源的遗传多样性和群体遗传结构。结果表明,利用从分布于大麦7个连锁群上的200对SSR引物中筛选的48对多态性引物,共检测到230个等位条带,变化范围为1~10个,平均每对SSR引物可检测到4.79个条带。多态性信息含量(PIC)变化范围为0.054 7~0.856 9,平均值为0.489 8。遗传相似系数(GS)的变化范围为0.469~0.924,平均值为0.745。经聚类分析,在GS约0.740处可将71份材料分为五大类,第51、43和14号品种分别聚为A、B和C类,第22、27、39和50号品种聚为D类,其余64个品种聚为E类,其中,第51号品种的穗长(3.8cm)、穗粒数(44.2粒)最低,第43号的单穗小穗数(90个)最高,但千粒重(35.6g)最低,第14号品种的生育期(86d)最短,但穗粒数(69.6粒)最多。此外,群体遗传结构分析表明,71份青稞资源材料可划分为2个亚群。In order to understand the genetic basis of hulless barley with cold tolerance from the Qinghai-Tibetan Plateau,genetic diversity and population genetic structure of 71 hulless barley germplasm resources from the Qinghai-Tibetan Plateau were analyzed using 48 SSR markers with better polymorphic bands,which were selected from 200 pairs of SSR primers located on the seven linkage groups of barley.The results showed that a total of 230 allelic bands were identified in 71 hulless barley materials,varying from 1 to 10 with the average alleles per SSR locus of 4.79.The variation range of genetic similarity coefficients was 0.469~0.924 with the average of 0.745.The polymorphic information content （PIC） ranged from 0.054 7 to 0.856 9 with the mean of 0.489 8.These hulless barley materials could be clustered into two subgroups according to population genetic structure analysis.Cluster analysis based on UPGMA （Unweight pair group method using arithmetic averages） method indicated that 71 hulless barley accessions could be divided into five major groups at the genetic similarity coefficient （GS） level of 0.740,and the cultivar No.51,No.43 and No.14 were clustered into the Group A,B and C,respectively,and the cultivar No.22,No.27 and No.39 as well as No.50 were clustered as Group D,and the others as Group E including 64 hulless barley accessions.The cultivar No.51 from Group A had the lowest spike length （3.8 cm） and grain number per spike （44.2）,and the No.43 from Group B had the highest spikelet number per spike （90） and lowest 1 000-grain weight （35.6 g）,but the No.14 showed the shortest growth period （86 d） and maximum grain number per spike （69.6）.The analysis based on population genetic structure and cluster analysis unanimously revealed that the genetic basis existed in most of hulless barley materials used in this study was narrow.

关 键 词：青稞 遗传多样性 SSR 群体结构 聚类分析 

分 类 号：S512.1[农业科学—作物学] S332