乳腺癌中BCL-6和ZEB2的表达及其意义  

Expression and significance of BCL-6 and ZEB2 in breast cancer

作　　者：郑丽[1] 赵敏[1] 昂琳[1] 黄金[1] 王瑾[1] 邹强[1] 吴正升[2] 

机构地区：[1]合肥市第二人民医院病理科,合肥230011 [2]安徽医科大学病理学教研室,合肥230032

出　　处：《临床与实验病理学杂志》2017年第1期50-54,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：合肥市自主创新政策"借转补"项目[合科(2014)71号]

摘　　要：目的探讨BCL-6和ZEB2在乳腺癌侵袭、转移及预后中的生物学意义。方法分别采用免疫组化SP两步法与原位杂交法检测228例乳腺癌和80例乳腺良性病变组织中BCL-6、ZEB2蛋白及mRNA的表达。结果乳腺癌组织中BCL-6、ZEB2蛋白及mRNA表达水平均显著高于乳腺良性病变组织(P<0.05);BCL-6表达与肿瘤大小、淋巴结转移、组织学分级、TNM分期及HER-2表达均呈正相关(P<0.05);ZEB2表达与肿瘤大小、淋巴结转移、TNM分期及HER-2表达均呈正相关(P<0.05);乳腺癌中BCL-6、ZEB2阳性者总生存期及无复发生存期均显著低于阴性者(P<0.01)。结论 BCL-6、ZEB2与乳腺癌演进过程密切相关,检测BCL-6和ZEB2的表达,有望成为监测与预警乳腺癌侵袭、转移及预后的重要手段。Purpose To explore the biological significance of BCL-6 and ZEB2 in invasion, metastasis and prognosis of breast cancer. Methods The expressions of BCL-6, ZEB2 pro- tein and mRNA were detected respectively in 228 cases of breast cancer and 80 cases of breast benign lesions by immunohisto- chemical SP two-step staining and situ hybridization. Result The expression levels of BCL-6, ZEB2 protein and mRNA in breast cancer tissues were significantly higher than in breast be- nign lesions （P 〈 0.05）. The expressions of BCL-6 was posi- tively correlated with tumor size, lymphatic metastasis, histolog- ical grade, TNM staging and HER-2 expression （P 〈 0.05）.The expressions of ZEB2 was positively correlated with tumor size, lymphatic metastasis, TNM staging and HER-2 expression （P 〈 0.05 ）. The overall survival and relapse-free survival of BCL-6 and ZEB2 positive expression were significantly less than the negative expression （ P 〈 0.01 ）. Conclusion The BCL-6 and ZEB2 are closely correlated with the evolution process of breast cancer, which may become important means for monito- ring and warning the metastasis, invasion, and prognosis of breast cancer.

关 键 词：乳腺肿瘤 BCL-6 ZEB2 免疫组织化学 原位杂交 

分 类 号：R737.9[医药卫生—肿瘤]