低温对大鼠全脑缺血再灌注时脑组织动力相关蛋白1表达的影响  

Effect of hypothermia on expression of dynamin-related protein 1 in brain tissues during global cere- bral ischemia-reperfusion in rats

作　　者：范婧婧[1] 惠康丽[1] 徐苗苗[1] 蔡慎权 仲浩[1] 操良斌 段满林[1] 徐建国[1] 

机构地区：[1]南京大学医学院附属金陵医院麻醉科,210000

出　　处：《中华麻醉学杂志》2016年第11期1397-1399,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81671884）

摘　　要：目的评价低温对大鼠全脑缺血再灌注时脑组织动力相关蛋白1（Drp 1）表达的影响。方法健康清洁级雄性SD大鼠36只，体重280～320 g，采用随机数字表法分为3组（n＝12）：假手术组（S组）、全脑缺血再灌注组（I/R组）和低温组（H组）。I/R组和H组采用经食道心脏起搏诱发心跳骤停＋心肺复苏方法制备大鼠全脑缺血再灌注损伤模型；H组于再灌注即刻体表降温，15 min之内将直肠温度降至32～34 ℃，采用自制变温箱维持6 h。于再灌注72 h时行神经功能缺陷评分，处死取脑组织，观察海马CA1区病理学改变，并行正常椎体细胞计数，采用TUNEL法检测神经元凋亡情况，计算神经元凋亡率，采用Western blot法检测海马组织Drp1和细胞色素c（Cyt c）的表达。 结果与S组比较，I/R组和H组神经功能缺陷评分和神经元凋亡率升高，正常椎体细胞计数降低，I/R组海马组织Drp1和Cyt c的表达上调（P〈0.05），H组Drp1和Cyt c的表达差异无统计学意义（P〉0.05）；与I/R组比较，H组神经功能缺陷评分和神经元凋亡率降低，正常椎体细胞计数升高，海马组织Drp1和Cyt c的表达下调（P〈0.05）。 结论低温抑制大鼠全脑缺血再灌注时细胞凋亡的机制可能与下调脑组织Drp1的表达有关。ObjectiveTo evaluate the effect of hypothermia on the expression of dynamin-related protein 1（Drp1）in brain tissues during global cerebral ischemia-reperfusion（I/R）in rats.MethodsThirty-six healthy male Sprague-Dawley rats, weighing 280-320 g, were divided into 3 groups（n=12 each）using a random number table： sham operation group（group Sham）, global cerebral I/R group（group I/R）and hypothermia group（group H）. Cardiac arrest was induced by transoesophageal cardiac pacing followed by cardiopulmonary resuscitation to establish the global cerebral I/R model in anesthetized rats in I/R and H groups.In group H, the body temperature（rectal temperature）was cooled down to 32-34 ℃ within 15 min starting from the beginning of reperfusion, and maintained at this level for 6 h. At 72 h of reperfusion, neurological deficit was scored, and the rats were sacrificed, and the whole brain was removed for examination of the pathological changes in hippocampal CA1 region and for determination of normal pyramidal cell count and neuronal apoptosis in hippocampal CA1 region and expression of Drp1 and cytochrome c（Cyt c）in hippocampal tissues（by Western blot）. The apoptosis rate was calculated.ResultsCompared with group S, the neurological deficit score and apoptosis rate were significantly increased, and the number of normal pyramidal cells was decreased in I/R and H groups, the expression of Drp1 and Cyt c in hippocampal tissues was significantly up-regulated in group I/R（P〈0.05）, and no significant change was found in the expression of Drp1 and Cyt c in hippocampal tissues in group H（P〉0.05）. Compared with group I/R, the neurological deficit score and apoptosis rate were significantly decreased, the number of normal pyramidal cells was increased, and the expression of Drp1 and Cyt c in hippocampal tissues was down-regulated in group H（P〈0.05）.ConclusionThe mechanism by which hypothermia inhibits cell apoptosis during global cerebral I/R may be related to down-regulation of Drp1

关 键 词：低温 人工 再灌注损伤 脑 动力蛋白质类 

分 类 号：R741[医药卫生—神经病学与精神病学]