前列腺癌ERG基因重排与蛋白表达及其与预后的关系  

Prognostic Significance of ERGGene Rearrangement and Protein Expression in Prostate Cancer

作　　者：潘秀懿 谭珺娅[1] 聂玲[1] 尹晓雪[1] 龚静[1] 陈雪芹[1] 周桥[1] 曾浩[2] 陈铌[1] 

机构地区：[1]四川大学华西医院病理科.病理研究室,成都610041 [2]四川大学华西医院泌尿外科,成都610041

出　　处：《四川大学学报（医学版）》2017年第1期66-70,共5页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(No.81272820;No.81272848;No.81572540和No.81572541)资助

摘　　要：目的检测中国前列腺癌患者ETS相关基因(ERG)重排及蛋白表达情况,分析其与患者预后的关系。方法选取四川大学华西医院2009~2014年前列腺穿刺确诊的前列腺癌患者482例,采用荧光原位杂交法检测ERG基因重排情况,采用免疫组化染色法检测ERG蛋白表达情况。采用Spearman秩相关分析ERG基因重排与蛋白表达的相关性,采用χ~2检验分析ERG基因重排和蛋白表达与患者临床病理特征的关系,采用KaplanMeier法分析ERG基因重排和蛋白表达与患者预后的关系。结果 87例(18.0%)患者检出ERG基因重排,其中45例(51.7%)为转位重排,42例(48.3%)为缺失重排。74例(15.4%)患者表达ERG蛋白。ERG蛋白表达与基因重排正相关(r=0.849,P=0.000)。368例获得随访资料,ERG基因重排状态及蛋白表达水平与患者年龄、Gleason评分、初诊时前列腺特异性抗原(PSA)水平无关(P>0.05),但发生远处转移及进展为去势抵抗性前列腺癌(CRPC)的病例中ERG蛋白表达率下降(P<0.05)。预后分析显示ERG基因重排及蛋白表达均不能提示预后。结论 ERG基因重排状态与蛋白表达情况均不能作为前列腺癌进展为CRPC及总生存率的独立预测指标。Objective To investigate gene rearrangement and protein expression of ETS related gene （ERG ） in prostate cancer of Chinese patients and its correlation with clinicopathological characteristics and prognosis. Methods This study collected 482 cases of prostatic adenocarcinomas diagnosed by prostate biopsy in West China Hospital of Sichuan University from 2009 to 2014. Fluorescencein situ hybridization （FISH） and immuno-histochemical staining （IHC） were performed to access the ERG rearrangement and protein expression respectively. Relationship between ERG rearrangement and protein expression was assessed by Spearman rank order correlation. The correlations of ERG rearrangement and protein expression with clinicopathological variables and prognosis were further analyzed. Results ERG rearrangement was detected in 87 （18.0 %） cases, of which 45 （51.7%） was translocation and 42 （48.3%） was deletion. ERG protein expression was detected in 74 （15.4%） cases. Follow-up data was obtained in 368 cases. ERG rearrangement and protein expression had no correlations to age, Gleason score and pre-operation PSA level （ P〉0.05）, but ERG protein level was decreased in metastatic cases or castration resistant prostate cancer （CRPC） cases （ P〈0.05）. Kaplan-Meier curve showed both gene rearrangement and protein expression of ERG had no prognostic significance. Conclusion ERG rearrangement, as well as ERG protein expression, could not serve as an independent prognostic biomarker.

关 键 词：前列腺癌 ERG 基因重排 蛋白表达 预后 

分 类 号：R737.25[医药卫生—肿瘤]