气相色谱法测定化妆品中苯酚和氢醌的可行性及误差分析  

Method feasibility and error analysis for phenol and hydroquinone determination in cosmetic by gas chromatography

作　　者：吴坤[1] 周贻兵[2] 张卫国[2] 王自布[1] 姜金仲[3] 

机构地区：[1]贵州师范学院化学与生命科学学院,贵阳550018 [2]贵州省疾病预防控制中心,贵阳550004 [3]贵州师范学院贵州省生物资源开发利用特色重点实验室,贵阳550018

出　　处：《微量元素与健康研究》2017年第1期59-61,共3页Studies of Trace Elements and Health

基　　金：生物资源科学专业综合改革试点项目(2012287);贵州省科学技术基金项目(黔科合J字[2013]2246号)

摘　　要：目的:探讨气相色谱测定化妆品中苯酚和氢醌的可行性,分析可能存在的误差来源及影响因素。方法:用气相色谱法FID检测器,以SE-30毛细管柱分离,误差分析方法按水质分析中的AQC工作程序进行。结果:苯酚和氢醌的最低定量浓度分别为0.58μg/g和2.02μg/g,线性范围苯酚为1.0~200μg/ml,氢醌为25~500μg/ml。0.2 c苯酚标准溶液、苯酚试样和加标苯酚的变异分析有非常显著差异(F>F_(0.01)),0.2 c氢醌标准溶液差异非常显著(F>F_(0.01)),氢醌试样和加标氢醌变异不显著(F<F0.01),除氢醌加标试样的总标准差(St)>浓度的5%(w),其他试样的St均<浓度5%(w),苯酚和氢醌试样准确度的置信限(R/d)为1.03和1.01,平均回收R能被接受。结论:方法的灵敏度较高,线性范围符合要求,试样及标准溶液的变异主要误差来源是苯酚和氢醌的不稳定所带来的随机因素而致,应控制其试样保存温度、放置时间以及光的照射等条件,才能确保测定结果理想的精密度和足够的准确度。Objective：Investigate method feasibility of phenol and hydroquinone determination in cosmetic using gas chromatography and analyse possible error source together with influencing factors. Methods： Phenol and hydroquinone in cosmetic was separated by SE- 30 capillary column and determined by gas chromatography with FID detector,data error was valued by AQC control procedure with water quality analysis for reference. Results： Minimum quantitative concentration of phenol and hydroquinone were 0. 58 and 2. 02 μ g/g respectively. The linear range of phenol was 1 ~ 200 μ g/ml and hydroquinone 25 ~ 500 μ g/ml. There was significant difference（ F 〉F0. 01） on mutation analysis between 0. 2C phenol standard solution,sample and spiked sample. Diversity of 0. 2c hydroquinone standard solution was significant difference（ F 〉F0. 01） whlie hydroquinone and spiked hydroquinone samples was not significant difference. Except standard added hydroquinone,standard deviations for samples was greater than 5% concentration（ W）. accuracy confidence limit（R‘d） for phenol and hydroquinone samples was 1. 03 and 1. 01,the average recovery of R can be received. Conclusion： The method was ensured with high sensitivity method and reliable linear range,instability of phenol and hydroquinone caused by random factors was the main error source of variation between sample and standard solution,we should control sample preservation temperature,standing time and light irradiation conditions to ensure the ideal precision and accuracy of determination rusults.

关 键 词：化妆品 苯酚 氢醌 误差 可行性 气相色谱 

分 类 号：O657.71[理学—分析化学]