长链非编码RNA NBAT1表达下调对膀胱癌5637细胞生物学行为的影响  

Effect of the downregulation of long non-coding RNA NBAT1 expression on biological behavior of bladder cancer 5637 cells

作　　者：邵凤平 王志平[1] 

机构地区：[1]兰州大学第二医院泌尿外科研究所,甘肃省泌尿系统疾病研究重点实验室,甘肃省泌尿系统疾病临床医学中心,甘肃兰州730000

出　　处：《肿瘤》2017年第1期19-26,共8页Tumor

基　　金：国家自然科学基金资助项目(编号:81372733)~~

摘　　要：目的 :检测长链非编码RNA成神经细胞瘤相关转录本1(neuroblastoma associated transcript 1,NBAT1)在人膀胱癌细胞株中的表达情况,并探讨NBAT1基因表达下调对膀胱癌5637细胞生物学行为的影响。方法:采用实时荧光定量PCR法检测7种膀胱癌细胞株(RT4、5637、EJ、J82、TCC-SUP、T24和UM-UC-3)中NBAT1 m RNA的表达水平,分析其与膀胱癌细胞恶性程度的关系。将靶向NBAT1基因的特异性si RNA转染膀胱癌5637细胞,实时荧光定量PCR法检测NBAT1基因的表达变化。然后,分别采用CCK-8法、细胞划痕愈合实验、Transwell小室实验和Hoechst染色法检测NBAT1基因表达下调对膀胱癌5637细胞增殖、迁移、侵袭及顺铂诱导后细胞凋亡的影响。结果 :NBAT1在恶性程度较低的膀胱移行细胞乳头瘤细胞RT4中的表达水平明显高于恶性程度较高的膀胱移行上皮癌细胞5637、EJ、J82、TCCSUP、T24和UM-UC-3(P值均<0.01)。特异性si RNA转染膀胱癌5637细胞后,NBAT1基因表达被明显抑制(P<0.01),5637细胞的增殖、迁移及侵袭能力均明显增强(P值均<0.01),然而顺铂诱导后的5637细胞凋亡没有明显变化(P>0.05)。结论 :NBAT1表达水平可能与膀胱癌细胞的恶性程度有关。下调NBAT1基因的表达可以增强膀胱癌5637细胞的增殖活力,并促进细胞迁移及侵袭。Objective： To investigate the expression of long non-coding RNA neuroblastoma associated transcript 1 （NBAT1） in human bladder cancer cells, and to explore the effect of downregulation of NBAT1 gene expression on the biological behavior of bladder cancer 5637 cells Methods： The mRNA expression level of NBAT1 in seven cell lines of human bladder cancer （RT4, 5637, EJ, J82, TCC-SUP, T24, and UM-UC-3） was detected by real-time fluorescent quantitative PCR （RFQ-PCR）, and the correlation of NBAT1 expression with the malignancy of bladder cancer cells was analyzed. The specific siRNA targeting NBAT1 gene was transfected into bladder cancer 5637 cells, and the change of NBAT1 gene expression was detected by RFQ-PCR. Then the effects of NBAT1 gene silencing on the proliferation, migration, invasion and cisplatin-induced apoptosis of 5637 cells were analyzed by using CCK-8 assay, wound healing assay, Transwell assay and Hoechst assay, respectively. Results： The expression level of NBAT1 mRNA in bladder cancer RT4 cells with lower invasive potential was significantly higher than that in bladder cancer cells （5637, EJ, J82, TCC-SUP, T24, and UM-UC-3） with higher invasive potential （all P 〈 0.01）. After the specific siRNA targeting NBAT1 gene was transfected into bladder cancer 5637 cells, the expression of NBAT1 gene was significantly inhibited （P 〈 0.01）. Furthermore, the proliferation, migration and invasion of 5637 cells were significantly promoted （all P 〈 0.01）, but cisplatin-induced apoptosis was not significantly changed after NBAT1 gene silencing （P 〉 0.05）. Conclusion： The expression level of NBAT1 gene may be correlated with the malignant degree of bladder cancer cells. Downregulation ofNBAT1 gene expression can improve the cell viability, and promote the migration and invasion of bladder cancer 5637 cells.

关 键 词：膀胱肿瘤 RNA 长链非编码 基因表达调控 肿瘤 细胞生 物学 成神经细胞瘤相关转录本1 

分 类 号：R737.14[医药卫生—肿瘤]