中国柑橘叶斑驳病毒和碎叶病毒发生状况及其分子特性研究  

Incidence and Molecular Characteristics of Citrus leaf blotch virus and Citrus tatter leaf virus Infecting Citrus in China

作　　者：项周[1,2] 程桥[1] 谢宗周[3] 王国平[1,2] 洪霓[1,2] 

机构地区：[1]华中农业大学植物科技学院,湖北省作物病害监测与安全控制重点实验室,武汉430070 [2]农业微生物学国家重点实验室,武汉430070 [3]华中农业大学园艺林学院,武汉430070

出　　处：《园艺学报》2017年第1期113-119,共7页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31272145);国家农业行业专项计划项目(nyhyzx200903017-08)

摘　　要：柑橘叶斑驳病毒(Citrus leaf blotch virus,CLBV)和柑橘碎叶病毒(Citrus tatter leaf virus,CTLV)均可侵染柑橘的不同种。为明确CLBV及CTLV在中国柑橘上的发生状况和分子特性,采用RT-PCR技术对来源于中国7个省的342份和346份柑橘样品进行检测,检出率分别为9.6%和11.0%。对14个CLBV分离物和15个CTLV分离物的外壳蛋白(Coat protein,CP)基因部分序列分析显示,CLBV分离物间cp核苷酸和编码氨基酸序列相似性分别为82.1%~100%和88.1%~100%,CTLV分离物的cp核苷酸和编码氨基酸序列相似性分别为89.5%~100%和92.5%~100%。测定的14个CLBV分离物在系统进化树中聚为2个组;而CTLV分离物分组不明显,该病毒的系统进化与寄主来源无明显关系。Both Citrus leaf blotch virus（CLBV）and Citrus tatter leaf virus（CTLV）can infect citrus species. To understand the infection status and molecular characteristics of the two viruses in citrus plants in China,citrus samples collected from seven provinces were detected by reverse transcription-polymerase chain reaction（RT-PCR）. Results showed that the incidences of CLBV and CTLV were 9.6% and 11.0%,respectively. The partial coat protein（cp）gene of 14 CLBV isolates shared 82.1%–100% sequence identities at nucleotide（nt）level and 88.1%–100% sequence identities at amino acid（aa）level,respectively. The partial cp gene of 15 CTLV isolates shared 89.5%–100% nt and 92.5%–100% aa sequence identities,respectively. Phylogenetic analyzes of partial cp gene sequences of both CLBV and CTLV showed that CLBV isolates identified clustered into two groups. The 15 CTLV isolates separated in different branches,and there is no clear relationships between their phylogenetic positions and original hosts.

关 键 词：柑橘叶斑驳病毒 柑橘碎叶病毒 检测 系统进化分析 

分 类 号：S666[农业科学—果树学]